Preparation method of porcine circovirus type 2 genetically engineered subunit oral vaccine

A technology of porcine circovirus and its manufacturing method, which is applied in the field of preparation of porcine circovirus type 2 genetically engineered subunit oral vaccine, can solve the problems of cumbersome vaccine process, stress vaccine, complex process, etc., and avoid antigen purification and optimization Protein expression conditions, the effect of increasing the probability of contamination

Active Publication Date: 2018-11-30
SHANDONG SINDER TECH
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Problems solved by technology

However, this method needs to purify the protein, and then add reagents such as inactivators and preservatives to emulsify the vaccine and carry out injection immunization. The process of preparing the vaccine is cumbersome and the process is complicated. As a routine injection vaccine, it is easy to cause stress and vaccine absorption problems.

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  • Preparation method of porcine circovirus type 2 genetically engineered subunit oral vaccine
  • Preparation method of porcine circovirus type 2 genetically engineered subunit oral vaccine
  • Preparation method of porcine circovirus type 2 genetically engineered subunit oral vaccine

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Embodiment Construction

[0028] In the following detailed description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, it will be understood by those skilled in the art that the present invention may be practiced without these specific details.

[0029] The present invention will be described in detail below with reference to the accompanying drawings and preferred embodiments.

[0030] In some illustrative embodiments, the preparation method of porcine circovirus type 2 genetically engineered subunit oral vaccine comprises the following steps:

[0031] Step 1. Optimizing and transforming the porcine circovirus type 2 ORF2 gene, and synthesizing the ORF2 gene sequence to obtain the pUC18-ORF2 plasmid;

[0032] Step 2, constructing the pMG36e-ORF2 plasmid in Escherichia coli for the pUC18-ORF2 plasmid, and performing sequencing analysis;

[0033] Step 3, constructing the pMG36e-ORF2 plasmid in Lactococcus lactis MG1363 to obtain ...

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Abstract

The invention relates to a preparation method of a porcine circovirus type II gene engineering subunit oral vaccine. The method comprises the following steps: 1, sequence synthesis; 2, construction of a recombinant vector in E. coli; 3, construction of a recombinant vector in Lactococcus lactis MG1363; and 4, protein expression, recombinant bacteria preservation experiment, and vaccine preparation. The invention has the following beneficial effects: no special treatment for the antigen avoids antigen purification and reduces antigen loss; optimization of the gene sequence improves the antigen expression; optimization of expression conditions solves the problems of toxicity to animals caused by addition of inducing agent for other prokaryotic expression and high costs, and avoids the increase of the chance of contamination and increase of the workload in the expression process. The method provided by the invention has low cost and significant effect; the invention employs oral vaccine to avoid the problems of stress and absorption caused by routine vaccination.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method of porcine circovirus type 2 genetic engineering subunit oral vaccine. Background technique [0002] Porcine circovirus type 2 (PCV2) can not only cause multisystemic wasting syndrome (PMWS) in weaned piglets, but also be associated with porcine dermatitis and nephrotic syndrome (PDNS), porcine respiratory disease syndrome (PRDC), and porcine congenital tremor. , sow reproductive disorders, pig hyperplasia and necrotizing pneumonia and other diseases are closely related. Since the discovery of the disease that can cause pigs in the 1890s, the PCV2 virus has spread rapidly in countries around the world. At present, the infection rate of PCV2 in pig herds is very high, which causes the growth of pigs to be slow and the feed remuneration to decrease. At the same time, it infringes on the immune system of pigs, leading to the decline of immune function of...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61P31/20C12N15/74C12N15/34C12R1/01
Inventor 李明义卢金凤刘阳李晓林赵航葛栋徐丽娜李佳棋李彦凤
Owner SHANDONG SINDER TECH
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