Preparation method of gibberellic acid GA3

A technology of gibberellic acid and seeds, applied in the field of preparation of high-purity gibberellic acid GA3, which can solve the problems of low purity of GA3, low extraction yield, and difficulty in solvent recovery

Active Publication Date: 2015-09-09
JIANGSU FENGYUAN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest weakness of this process is: the extraction yield is low, and the purity of GA3 is not high, and the solvent recovery after adsorption is also difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Take by weighing 100 grams of gibberellic acid GA3 coarse powder (the content of GA3 in this coarse powder is 85.0%), add acetone 500mL, adjust the pH to all dissolved with 3mol / L NaOH, filter, and dissolve with 5%H 2 SO 4 Adjust the pH of the acetone liquid dissolved with gibberellic acid GA3 to 3.0. With continuous stirring, a large amount of powder is precipitated, filtered and drained, the filter cake is rinsed with 100 mL of acetone, filtered and drained, and then washed twice with appropriate amount of distilled water , then rinsed with an appropriate amount of water, drained, and dried at 60-70° C. to obtain purified gibberellic acid GA3 solid. After testing, the purity of the gibberellic acid GA3 solid was 98.74%, and the yield was 98.1%. The acetone in the filtrate after several times of filtration is distilled under negative pressure, and then recycled.

Embodiment 2

[0041](1) GA3 bacterial classification is received on the seed culture medium that contains 3% starch, 0.05% glucose, 0.3% peanut cake powder, 0.5% soybean cake powder, 0.005% inorganic salt and cultivates, and on seed culture medium, temperature is Cultivate at 28°C for 6 days to obtain a suspension;

[0042] (2) Continue to cultivate the suspension obtained in step (1) for 3 days at a temperature of 28° C. in the seed tank, and then move it to the fermenter for 8 days at a temperature of 28 ± 1° C. Carry out fermentation, in fermentation process, add the nitrogen-containing mixture of 30mg / ml (in terms of nitrogen element), control the viscosity of fermented liquid at 10-20mPa. Concentrate and extract to obtain mixed dry powder A.

[0043] (3) Weigh 100 grams of the mixed dry powder A of step (2) (the content of GA3 in the dry powder is more than 85%), add 500 mL of acetone, adjust the pH with 2.5mol / L NaOH to completely dissolve, filter, and dissolve the powder with 5% H ...

Embodiment 3

[0045] (1) Connect the GA3 strain to the seed medium containing 3% starch, 0.05% glucose, 0.5% maltose, 0.2% dextrin, 0.3% peanut cake powder, 0.5% soybean cake powder, and 0.005% inorganic salt for cultivation , culturing on the seed medium at a temperature of 28° C. for 6 days to obtain a suspension;

[0046] (2) Continue to cultivate the suspension obtained in step (1) for 2 days at a temperature of 28° C. in the seed tank, and then move it to a fermenter for 9 days at a temperature of 28 ± 1° C. Carry out fermentation, in fermentation process, add the nitrogen-containing mixture of 30mg / ml (in terms of nitrogen element), control the viscosity of fermented liquid at 10-20mPa. Concentrate and extract to obtain mixed dry powder A;

[0047] (3) Weigh 100 grams of the mixed dry powder of step (2) (the content of GA3 in the dry powder is 85%)) and add 500 mL of acetone, adjust the pH to dissolve completely with 4.5mol / L NaOH, filter, and dissolve the mixture with 5% H 2 SO 4 ...

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Abstract

The invention provides a preparation method of gibberellic acid GA3. The preparation method comprises the following steps: (1) inoculating GA3 strains onto a culture medium, and culturing so as to obtain a suspension liquid; (2) further culturing the suspension liquid obtained in the step (1) in a seed tank, then inoculating the suspension liquid into a fermentation tank, fermenting so as to obtain a fermentation liquor, and treating the fermentation liquor to obtain mixed dry powder A; and (3) adding the mixed dry powder obtained in the step (2) into acetone, further adding an alkaline solution until the dry powder is completely dissolved, then adding an acid solution to regulate the PH of the solution to be 2.5-3.5, stirring so as to separate out a solid, and performing filtering, washing and drying treatment, thereby obtain a gibberellic acid GA3 solid. The method has the advantages that the gibberellic acid GA3 purity is improved, the product purity is not less than 95%, and the total extraction yield is not less than 85%.

Description

technical field [0001] The invention relates to a preparation method of gibberellic acid, in particular to a preparation method of high-purity gibberellic acid GA3. Background technique [0002] Gibberellic acid GA3 is a plant growth regulator, which is a high-efficiency, low-residue, and pollution-free biological pesticide. It can promote the growth of plant stems and leaves, reduce the shedding of buds and bolls in cotton, increase the yield of barley, grapes, citrus, pears, etc. Fruits increase fruit set rate. Especially in the aspects of hybrid rice seed production and promoting late rice heading, the medicinal effect is good and the dosage is large. There are only 3 domestic manufacturers of original drugs, while there are more than 20 manufacturers of preparations. In addition, the demand in the international market has been strong for many years, and the export volume has continued to rise. [0003] As early as the 1960s, Britain, the United States, and the Soviet U...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/93
CPCC07D307/93
Inventor 徐直周小东
Owner JIANGSU FENGYUAN BIOENG
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