Preparation method of titanium disulfide nanosheet/quantum dot composite mercury ion fluorescent probe

A titanium disulfide, fluorescent probe technology, applied in the intersection of chemistry, physics and materials science, to achieve the effect of high sensitivity, simple equipment and broad application prospects

Active Publication Date: 2016-03-30
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In summary, although the relevant Hg 2+ The preparation method of fluorescent probe has been reported in the literature, but so far there is no Hg based on titanium disulfide nanosheet / quantum dot composite as the main material. 2+ Domestic and foreign literature and patent reports of fluorescent probes

Method used

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  • Preparation method of titanium disulfide nanosheet/quantum dot composite mercury ion fluorescent probe
  • Preparation method of titanium disulfide nanosheet/quantum dot composite mercury ion fluorescent probe
  • Preparation method of titanium disulfide nanosheet/quantum dot composite mercury ion fluorescent probe

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Embodiment 1

[0034] Weigh 20mgTiS 2 The powder was dispersed in 5mL N-methylpyrrolidone, and ultrasonically treated for 1h to form a suspension. Centrifuge the sonicated suspension and collect the upper dispersion to remove larger particles. Pour the upper dispersion liquid into the flask, and stir the reaction with magnetic force at 100°C for 6h to obtain TiS 2 QDs / nanosheet composite solution. The complex solution was centrifuged, and the upper dispersion was collected as TiS 2 QDs, the lower precipitate is TiS 2 Nanosheets. Add 0.1M phosphate buffer (pH=7.0) to the prepared upper layer dispersion, stir and react at room temperature for 15min, then add 10μLODN (20μM), incubate at room temperature for 12h, and then dialyze in a dialysis bag to remove unreacted TiS 2 QDs, prepared ODN surface-modified TiS 2 QDs. ODN surface modified TiS 2 QDs dispersion and TiS2 Nanosheet dispersion mixed, where TiS 2 QDs and TiS 2 The mass ratio of the nanosheets is 1:2, incubate at room temper...

Embodiment 2

[0037] Weigh 30mgTiS 2 The powder was dispersed in 5mL N-methylpyrrolidone, and ultrasonically treated for 2h to form a suspension. Centrifuge the sonicated suspension and collect the upper dispersion to remove larger particles. Pour the upper dispersion liquid into the flask, and stir the reaction with magnetic force at 120°C for 6h to obtain TiS 2 QDs / nanosheet composite solution. The complex solution was centrifuged, and the upper dispersion was collected as TiS 2 QDs, the lower precipitate is TiS 2 Nanosheets. Add 0.1M phosphate buffer (pH=8.0) to the prepared upper layer dispersion, stir and react at room temperature for 15min, then add 20μLODN (20μM), incubate at room temperature for 24h, and then dialyze in a dialysis bag to remove unreacted TiS 2 QDs, prepared ODN surface-modified TiS 2 QDs. ODN surface modified TiS 2 QDs dispersion and TiS 2 Nanosheet dispersion mixed, where TiS 2 QDs and TiS 2 The mass ratio of nanosheets is 1:5, incubate at room temperat...

Embodiment 3

[0040] Weigh 50mgTiS 2 The powder was dispersed in 5mL N-methylpyrrolidone, and treated with ultrasound for 5h to form a suspension. Centrifuge the sonicated suspension and collect the upper dispersion to remove larger particles. Pour the upper dispersion liquid into a flask, and react with magnetic stirring at 150°C for 6 hours to obtain TiS 2 QDs / nanosheet composite solution. The complex solution was centrifuged, and the upper dispersion was collected as TiS 2 QDs, the lower precipitate is TiS 2 Nanosheets. Add 0.1M phosphate buffer (pH=9.0) to the prepared upper layer dispersion, stir and react at room temperature for 15min, then add 50μLODN (50μM), incubate at room temperature for 36h, and then dialyze in a dialysis bag to remove unreacted TiS 2 QDs, prepared ODN surface-modified TiS 2 QDs. ODN surface modified TiS 2 QDs dispersion and TiS 2 Nanosheet dispersion mixed, where TiS 2 QDs and TiS 2 The mass ratio of nanosheets is 1:10, incubate at room temperature ...

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Abstract

The invention relates to a preparation method of a titanium disulfide nanosheet / quantum dot composite mercury ion fluorescent probe, which comprises the following steps: hydrothermal synthesis is carried out on a raw material titanium disulfide to prepare lamellar-structure TiS2 nanosheets, and an ultrasonic-assisted chemical process is carried out to prepare TiS2 fluorescent quantum dots; a terminal amino group of single-chain oligodeoxyribonudeotide is combined with a carboxy group on the TiS2QDs surface stabilizer molecules to form ODN-modified TiS2QDs, wherein the ODN can be attached to the TiS2 nanosheets under the action of intermolecular force, so that the TiS2QDs indirectly contact the nanosheets to generate fluorescent resonance energy transfer, thereby causing TiS2QDs fluorescent quenching; Hg<2+> can be specifically combined with thymine base on the single chain ODN to form a T-Hg<2+>-T structure, so that the ODN departs from the TiS2 nanosheet surface, and the TiS2QDs also depart, thereby causing fluorescent restoration; and the relationship between the fluorescence intensity and Hg<2+> concentration of the system is fitted to construct the Hg<2+> fluorescent probe based on the titanium disulfide nanosheet / quantum dot composite. The method implements composite construction of the ionic fluorescent probe from the titanium disulfide nanosheet / quantum dot composite and single-chain ODN.

Description

technical field [0001] The invention belongs to the cross field of chemistry, physics and material science, and specifically relates to a method for preparing a titanium disulfide nanosheet / quantum dot composite mercury ion fluorescent probe. Background technique [0002] single-layer or multi-layer titanium disulfide (TiS 2 ) composed of TiS 2 Nanosheet is a new type of two-dimensional layered nanomaterial with graphene-like structure and properties. When its geometric size, especially its thickness, is reduced to the nanoscale range, due to the influence of quantum confinement effects, it will show A series of unique optical and electrical properties. Compared with graphene without bandgap, layered TiS 2 It has a direct bandgap and thus exhibits remarkable photofluorescence. In recent years, TiS 2 Based on their unique photoelectric properties, nanomaterials based on nanomaterials have shown broad application prospects in the fields of new energy, biosensing, photoele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/67G01N21/64
Inventor 王宗花朱欣欣桂日军金辉杨敏夏建飞
Owner QINGDAO UNIV
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