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Au/Ag nanoparticle dipolymer raman signal-based method for performing ultra-sensitive detection on folic acid

A gold nanoparticle and silver nanoparticle technology, applied in the field of analytical chemistry, can solve problems such as lack of high sensitivity, and achieve the effect of improving sensitivity and stabilizing detection limit

Active Publication Date: 2015-09-09
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The signal of traditional Raman scattering spectroscopy is weak, and it often lacks high sensitivity as a means of information readout

Method used

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  • Au/Ag nanoparticle dipolymer raman signal-based method for performing ultra-sensitive detection on folic acid
  • Au/Ag nanoparticle dipolymer raman signal-based method for performing ultra-sensitive detection on folic acid
  • Au/Ag nanoparticle dipolymer raman signal-based method for performing ultra-sensitive detection on folic acid

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0018] Example 1 Method for ultrasensitive detection of folic acid based on the Raman signal of gold-silver nanoparticle dimer

[0019] (1) Synthesis of 25nm gold nanoparticles and 10nm silver nanoparticles:

[0020] Synthesis of 25nm gold nanoparticles: add 195mL ultrapure water to a clean 250mL conical flask, add 5mL 0.4% chloroauric acid solution, stir and heat to boiling, add 3.2mL mass concentration after 7-8min It is a 1% trisodium citrate solution. After the solution changes from colorless to wine red, the heating is stopped and the stirring is continued until it is cooled to room temperature to obtain gold nanoparticles with a particle size of 25 nm.

[0021] Synthesis of 10nm silver nanoparticles: take 20mL of ultrapure water and 5mL of polyvinylpyrrolidone with a mass concentration of 1%, mix them in a conical flask, add 0.6mL of 0.1mol / L sodium borohydride aqueous solution, and perform ice bath protection. Then, 5mL of polyvinylpyrrolidone aqueous solution with a m...

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Abstract

The invention discloses an Au / Ag nanoparticle dipolymer raman signal-based method for performing ultra-sensitive detection on folic acid and belongs to the technical field of material chemistry. The method comprises the steps: synthesis of Au / Ag nanoparticles, modification of the Au nanoparticles and a folic acid antibody, modification of the Ag nanoparticles and a folic acid antigen, assembling of an Au / Ag nanoparticle dipolymer, and representation of TEM and raman signals; the folic acid is detected based on difference of the Au / Ag nanoparticle dipolymer raman signals. According to the method, the assembling of the Au / Ag nanoparticle dipolymer is performed by the specific binding of the antigen and the antibody, and the fast and ultra-sensitive detection on the folic acid can be realized based on the relatively high Au / Ag nanoparticle dipolymer raman signals.

Description

technical field [0001] The invention relates to a method for ultra-sensitive detection of folic acid based on a gold-silver nanoparticle dimer Raman signal, belonging to the technical field of analytical chemistry. Background technique [0002] Nanomaterials generally refer to materials whose structural units are between 1 and 100 nm in size. Due to their size, nanostructured materials have unique physical and chemical properties and become one of the most active fields of material research. Raman spectroscopy, as a kind of inelastic scattering spectrum, analyzes the vibration and rotation of molecules through the scattering spectrum with different frequencies from the incident light, and can provide information about various normal vibrational frequencies and related vibrational energy levels inside the molecule. Traditional Raman scattering spectroscopy has weak signals and often lacks high sensitivity as an information readout method. The discovery and confirmation of su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 徐丽广高凤丽胥传来匡华马伟刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV
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