Method for cell preservation by use of biocompatible particles

Abstract

The invention relates to a method for cell preservation by use of biocompatible particles. The method is characterized in that physical mixing and centrifugal enrichment are conducted on hydrogel particles of a pure biocompatible material and cells, and the hydrogel particles are stacked to wrap the cells. The method is also characterized in that hydrogel particles, added with magnetic nano-particles, of a pure biocompatible material and cells are subjected to physical mixing and then centrifugal enrichment, the magnetic hydrogel particles are stacked to wrap the cells, and when separation is required, the magnetic biocompatible particles are separated through magnetic separation to obtain the needed cells. The biocompatible material particles are 10 nm-1000 [mu]m, the compatible particles and the cells are uniformly mixed according to any appropriate proportion and subjected to centrifugal enrichment at 500-2000 rpm for storage. The pure physical method for particle-stacking cell preservation of the biocompatible material in the noncryogenic environment is adopted for the first time, so that a novel, simple, convenient and effective method is provided for cell preservation and transport at different temperatures and different bad degrees.

Description

technical field [0001] The invention belongs to the field of biomaterials, and specifically relates to a method for preserving cells by using biocompatible particles; in particular, it relates to a purely physical method that is harmless to cells under non-low temperature freezing conditions at different temperatures and different degrees of severity Methods of preserving and transporting living cells. Background technique [0002] With the development of biotechnology, the use of cells isolated from tissues for testing and detection is a very important part of biotechnology-related fields. The isolated cells are widely used in the detection of pathogens, disease detection, disease diagnosis, new drug testing, animal and plant quarantine, environmental pollutant testing and so on. However, the vast majority of cells are surrounded by three-dimensional tissue matrix in vivo, such as figure 1 a, From a microscopic point of view, the macromolecules of the tissue matrix need t...

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Problems solved by technology

[0004] The purpose of the present invention is to use the particles of biocompatible materials for the first time in view of the cumbersome and inefficient cell preservation methods in the prior art and the shortage of cell transportation methods due to the rapid death of cells after being separated from the living tissue package. The physical method packs and wraps the cells, and creates a three-dimensional environment simulating the tissue matrix for the cells to wrap the cells under non-cryopreservation conditions, so that the cells feel as if they are still in a "protected" safe environment in the tissue, effectively using Cell survival, in addition, whether this method is to wrap cells or separate cells, it is a pure physical process that does not need to add toxic chemicals, providing a simple and efficient non-low temperature preservation method; at the same time, at different temperatures (normal temperature, physiological temperature) etc.), different carbon dioxide concentrations (5%, atmospheric concentration, etc.) also have strong storage capacity, providing a suitable and convenient cell transport method

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