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Molecular marker for diagnosis and treatment of intrauterine fetal growth restriction

An endogenous and fetal technology, applied in the field of biomedicine, can solve the problems of decreased gene expression, increased methylation level, and increased risk of type 2 diabetes in IUGR patients

Active Publication Date: 2015-09-23
BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

Studies have reported that the methylation level of the pancreatic duodenum homeobox gene-1 (pancreatic duodenum homeobox gene-1, Pdx1) gene promoter region in IUGR rats was significantly increased, the expression of Pdxl gene decreased, and type 2 increased in IUGR patients. risk of developing diabetes

Method used

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  • Molecular marker for diagnosis and treatment of intrauterine fetal growth restriction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Screening of differentially methylated genes

[0036] 1. Sample collection: From January 1, 2013 to June 30, 2013, infants with intrauterine growth restriction (IUGR) hospitalized in the Department of Pediatrics of Beijing Friendship Hospital and infants appropriate for gestational age (appropriate for gestational age, AGA) clinical data and blood samples. Each patient signed an informed consent, and the study was approved by the Beijing Municipal Ethics Committee.

[0037] Clinical data: 8 cases of IUGR, 4 cases of AGA, there was no difference in gender between the two groups, gestational age and birth weight were expressed as median (minimum value, maximum value), and there was no special maternal pregnancy period. The control group were infants suitable for gestational age who were admitted to the hospital at the same time. They were mostly admitted to the hospital due to jaundice, neonatal wet lung and other problems, without other major diseases. Some in...

Embodiment 2

[0053] Example 2 Verification of Differentially Methylated Genes

[0054] In order to clarify whether the chip data is reliable, we collected 40 cases of IUGR children and 40 cases of AGA, and selected the differentially methylated gene PI3KR1, and used MassARRAY Compact System (purchased from Sequenom Company) for verification. The specific operation was in accordance with the instruction manual. conduct. In simple terms it looks like this:

[0055] 1. The extraction of gDNA was carried out according to the steps in Example 1.

[0056] 2. Sulfite modification was carried out according to the steps in Example 1.

[0057] 3. PCR amplifies the chr5:67585758-675867575' fragment, and the amplification primers are as follows:

[0058] 5' end primer:

[0059] aggaagagagTGTTTTTTTTAATGTGGTATATTTGTGA;

[0060] 3' end primer:

[0061] cagtaatacgactcactatagggagaaggctAAAAAATATCACCAAACCACTCCTAC.

[0062] 4. Use the Mass CLEAVE Kit kit for alkaline phosphatase treatment, and then use...

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Abstract

The present invention discloses application of a hypomethylation gene PIK3R1. On the one hand, the hypomethylation gene PIK3R1 can be used to the preparation of diagnostic products for intrauterine fetal growth restriction, and on the other hand the hypomethylation gene PIK3R1 can be used to prepare the medicament for treating intrauterine fetal growth restriction. The experiments of the present invention demonstrate that the patients with intrauterine fetal growth restriction have PIK3R1 gene hypomethylation in blood; the findings provide strong for molecular biology basis for the diagnosis and treatment of patients with intrauterine fetal growth restriction, and have far-reaching clinical significance and generalization value.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a molecular marker for the diagnosis and treatment of fetal intrauterine growth restriction, in particular to the application of PIK3R1 gene methylation and its detection reagent in the preparation of fetal intrauterine growth restriction diagnostic products and therapeutic drugs. Background technique [0002] Intrauterine growth restriction (IUGR), also known as intrauterine growth retardation (IUGR), refers to the birth weight below the 10th percentile or lower than the average fetal weight of the corresponding gestational age. It is one of the main perinatal complications, and the mortality rate of perinatal infants is 8 times that of normal infants. It is estimated that approximately 30 million IUGR newborns are born each year in developing countries. The sequelae of IUGR are mainly divided into short-term sequelae and long-term sequelae after birth. The short-term sequelae of IUGR a...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A61K45/00A61P15/00A61P3/00
CPCA61K45/00C12Q1/6883C12Q2600/154
Inventor 丁瑛雪崔红
Owner BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV
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