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Quick biological sample detection method

A detection method and technology for biological samples, applied in the field of medical rapid diagnosis and detection, can solve the problems of difficulty in the accuracy and precision of detection results, residues, insufficient response, etc., to achieve improved accuracy and precision, complete information, The effect of low background signal

Inactive Publication Date: 2015-09-30
上海执诚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the chromatography process, the sample will remain on the reagent card and the reaction is not sufficient, so it is difficult to guarantee the accuracy and precision of the test results, so in most cases it is only used for qualitative or semi-quantitative detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In order to describe the content of the present invention more clearly, the following takes C-reactive protein (CRP) as an example for further description. Double-antibody sandwich method, 1000nm carboxy-modified magnetic beads, europium-chelated fluorescent particles, and anti-CRP antibodies 1 and 2. Antibody 1 and antibody 2 used mouse anti-human MC01 antibody and MC02 antibody of Hangzhou Qitai Biotechnology, respectively.

[0018] The binding of CRP antibody 1 to magnetic beads can be prepared by the following method:

[0019] (1) Washing and dispersion of magnetic beads: wash with 50mmol / l MES buffer and disperse in MES buffer.

[0020] (2) Activation of magnetic beads: Weigh activator EDC and NHS and dissolve in (1) magnetic bead solution, the concentration of activator is 100mg / ml, react for 10 minutes. Wash and disperse with MES buffer, and the concentration of activated magnetic beads is 10mg / ml.

[0021] (3) Connection between magnetic beads and antibody: t...

Embodiment 2

[0031] In order to describe the content of the present invention more clearly, the N-terminal pro-brain natriuretic peptide (NT-proBNP) detection item is taken as an example for further description below. Using double-antibody sandwich method, 200nm carboxy-modified magnetic beads, europium-chelated fluorescent particles (excitation light is about 300nm, emission light is about 600nm), antibody 1 uses NT‐proBNP produced by Hytest company with batch number: 13 / 01-8NT2 Antibody; Antibody 2 using the batch number produced by Hytest Company: 13 / 02-NT1-G12 anti-antibody.

[0032] The combination of NT-proBNP antibody 1 and magnetic beads is the same as Example 1:

[0033] The combination of NT-proBNP antibody 2 and fluorescent particles is the same as in Example 1.

[0034] The composition of the sample treatment solution in the NT‐proBNP in vitro diagnostic kit: Tris‐HCl50mmol / l), Casein (0.5%), Tween‐20 (1%), magnetic beads combined with NT‐proBNP antibody 1, and NT‐proBNP antib...

Embodiment 3

[0037] In order to describe the content of the present invention more clearly, the procalcitonin (PCT) detection item is taken as an example for further description below. Double-antibody sandwich method, 200nm carboxy-modified magnetic beads, AMCA fluorescent particles from Thermo Fisher (excitation light is about 340nm, emission light is about 440nm), antibodies are mouse anti-human MJG03 antibody and MJG05 from Hangzhou Qitai Biotechnology Antibody.

[0038] The combination of PCT antibody 1 and magnetic beads is the same as Example 1:

[0039] The combination of PCT antibody 2 and fluorescent particles is the same as in Example 1.

[0040]The composition of the sample treatment solution in the PCT in vitro diagnostic kit: Tris‐HCl 50mmol / l), Casein (0.5%), Tween‐20 (1%), magnetic beads combined with PCT antibody 1, and fluorescent particles combined with PCT antibody 2.

[0041] The analysis process is as follows, add 90ul sample to 10ul sample treatment solution, then m...

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Abstract

The invention provides a quick biological sample detection method which is characterized in that a detection sample is treated by a sample treatment liquid so as to form a magnetic bead, detected object and luminous marked micro particle magnetic bead composite; the treated detection sample is dropped on a biological chip; the detection sample flows to the other side along a capillary on the chip; under the action of a positioning magnetic field, the magnetic bead composite is gathered in a capillary detection region; an exciting light element generates exciting light with a certain wavelength to irradiate the detection region on the capillary; luminous marked micro particles on the composite generate emitting light; and a light detection element converts the intensity of the emitting light into a digital signal to form a detection result. Compared with a conventional lateral flow chromatography detection method, the quick biological sample detection method disclosed by the invention has the advantages of high sensitivity, wide detection range, high interference resistance, short reaction time and more suitability for being applied to quick detection on the biological sample.

Description

technical field [0001] The invention belongs to the technical field of medical rapid diagnosis and detection, in particular to an analysis method for rapid diagnosis of biological samples. Background technique [0002] Immunological determination is a quantitative technique based on the specific recognition of antibody-antigen, antibody labeling technology and tracer technology. Existing clinical in vitro diagnostic methods include radioimmunoassay, enzyme-linked immunoassay, time-resolved fluorescence immunoassay, chemiluminescence assay and so on. However, in the actual immunoassay, due to the large amount of impurities contained in the sample to be tested, the detection speed, detection sensitivity and accuracy are affected to a certain extent, so the target analyte can be quickly separated and purified from the complex sample matrix. , is a difficult problem faced by biological sample testing. This problem is especially prominent when used in the field of rapid diagnos...

Claims

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Application Information

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IPC IPC(8): G01N21/63G01N21/64
Inventor 王军峰王辉吴静
Owner 上海执诚生物科技有限公司
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