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Method for extracting eulexine from aboveground parts of thermopsis lanceolate

A technology for lanceolate rhizoma and genistein, which is applied in the field of separation and extraction of plant effective components, and achieves the effects of large processing capacity of raw materials, high genistein exchange and content improvement.

Inactive Publication Date: 2015-10-07
XIAN BOTANICAL GARDEN SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of method that extracts cytisine from the aerial part of lanceolus Huanghua, and the present invention overcomes the problem that the existing method for extracting cytisine is not suitable for taking the aerial part of lanceolate Huanghua as raw material, and has the advantages of Easy to operate, less organic solvent consumption, lower cost, easy industrial production, etc.

Method used

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  • Method for extracting eulexine from aboveground parts of thermopsis lanceolate
  • Method for extracting eulexine from aboveground parts of thermopsis lanceolate
  • Method for extracting eulexine from aboveground parts of thermopsis lanceolate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0026] Embodiment 1-3: Comparison of different sample loading liquid volumes on the extraction effect of cytisine

[0027] Take 100 kg of dried lanceolus Huanghua aerial parts, extract 3 times with 80% methanol under heat reflux, combine the extracts, filter, concentrate under reduced pressure, adjust the pH to about 2 with hydrochloric acid, extract 2 times with an equal amount of ethyl acetate, The extracted acidic aqueous solution was concentrated under reduced pressure to remove ethyl acetate to obtain the extract of Coniferus chinensis. The pH of the above extract was adjusted to 12 with NaOH aqueous solution, and the content of cytisine was 23.05 mg / mL to obtain the sample solution of lanceolata Huanghua, which was further divided into 1 / 10, 3 / 10 and 3 / 10 of the original volume. / 5, respectively passed through the treated different hydrogen 001×7 cation exchange resin columns at the flow rate of 1.0, 1.5 and 2.0BV / h. ), eluted with 1N ammonia water / 90% ethanol, until th...

Embodiment 4

[0029] Embodiment 4: HPLC analysis of the purity of cytisine extract

[0030] Chromatographic conditions:

[0031] Chromatographic column: UltisilTM Wel-C8 (5μm, 4.6mm×250mm); mobile phase: methanol-0.05mol / L potassium dihydrogen phosphate aqueous solution (15:85); volume flow rate: 1.0ml / min; detection wavelength: 314nm, column Temperature: 25°C.

[0032] Preparation of reference solution:

[0033] Accurately weigh an appropriate amount of cytisine and N-formyl cytisine, which were dried to constant weight at 60°C, respectively, put them in a 50mL measuring bottle, dissolve them to the mark with mobile phase, and make each 1mL containing cytisine and N -The reference substance solution of 0.36 mg and 0.22 mg of formyl pedistine respectively.

[0034] Linear Relationship Examination

[0035] Precisely draw 0.10, 0.25, 0.5, 1.25, 2.5mL of the above-mentioned reference solution solution and place them in a 10mL measuring bottle, dilute to the mark with mobile phase, mix well...

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Abstract

The invention discloses a method for extracting eulexine from aboveground parts of thermopsis lanceolate. A specific cationic resin is adopted as a major separation carrier to extract the eulexine extract. The raw materials adopted in the method refer to the aboveground parts of the thermopsis lanceolate at the flowering phase, and the fluid extract of the aboveground parts of the thermopsis lanceolate can be obtained by virtue of conventional methanol thermal reflux extraction and concentration. The extract is dissolved in an inorganic acid and then filtered; the pH value of the filtrate is approximately regulated to the range of 8-12 by use of ammonia water and the like; after partial impurities are removed by virtue of chromatographic separation by using the cationic resin, the filtrate is eluted by use of ammoniacal ethanol, and then the eluate containing the eulexine is concentrated and dried to obtain the eulexine extract, wherein the content of the eulexine can be above 43%. The method is used for overcoming the problem that the existing method for extracting the eulexine is not available for taking the aboveground parts of the thermopsis lanceolate as the raw materials, and the method has the characteristics of simple and convenient operation, low dosage of organic solvents, relatively low cost, easy industrial production and the like.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of plant effective components, and in particular relates to a method for extracting cytisine from the aerial part of lanceolata chinensis. Background technique [0002] Cytisine is a kind of 4 beta 2 The nicotinic acetylcholine receptor has a high affinity partial agonist, and it is also a relatively low price and effective smoking cessation ingredient. [0003] Although the seed part of lanceolus Huanghua is the traditional raw material for extracting and separating cytisine, because this plant is a wild species, the yield of seeds is greatly affected by the climate and its own growth physiological conditions, so it is not suitable as a raw material for large-scale industrial production. And this kind of plant is a perennial herb, which is the main pasture dominant species in the northern grassland, rich in resources, and also the main poisonous plants that endanger animal hus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/22
CPCC07D471/22
Inventor 周军辉李杨余刚卫伟光刘立成
Owner XIAN BOTANICAL GARDEN SHAANXI PROV