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Ruditapes philippinarum anti-oxidation peptide and preparation method thereof

A technology of Philippine clams and antioxidant peptides, applied in the field of food biology, can solve the problems of restricting the market development of Philippine clams processed products, low product added value, etc., and achieve the effects of low price, considerable appreciation and wide sources.

Inactive Publication Date: 2015-10-14
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the processing of Philippine clams in my country at this stage is mainly based on traditional products, and the added value of products is low, which seriously restricts the market development of processed products of Philippine clams.

Method used

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  • Ruditapes philippinarum anti-oxidation peptide and preparation method thereof
  • Ruditapes philippinarum anti-oxidation peptide and preparation method thereof
  • Ruditapes philippinarum anti-oxidation peptide and preparation method thereof

Examples

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Effect test

Embodiment 1

[0029] (1) Enzymatic hydrolysis preparation of antioxidant peptides: After washing the live Filipino clams with water, remove the shells, take the meat, and make a pulp. Add a phosphate buffer solution with a pH value of 7.5 and a concentration of 0.01mol / L according to the ratio of material to liquid 1:5, add trypsin 6000U / g at 50°C for 3.5 hours, inactivate the enzyme at 100°C for 5min, and the obtained The enzymolyzate was centrifuged at 8000rpm for 10min, and the obtained supernatant was the clam enzymolyzate;

[0030](2) Separation and purification of antioxidant peptides: the enzymolysis solution was separated by Sephadex G-25 gel chromatography, using pure water as the eluent, detected at 280nm, the flow rate was 2mL / min, and the antioxidative activity of each component was detected. Oxidation activity, collect the RPTE2 component with the highest activity, freeze-dry it and make it into a 50mg / mL solution with deionized water, and then use Sephadex G-15 gel chromatogra...

Embodiment 2

[0033] (1) Enzymatic hydrolysis preparation of antioxidant peptides: After washing the live Filipino clams with water, remove the shells, take the meat, and make a pulp. Add a phosphate buffer solution with a pH value of 7.5 and a concentration of 0.01mol / L according to the ratio of material to liquid 1:4, add trypsin 5400U / g at 55°C for 3 hours, and then inactivate the enzyme at 95°C for 5min, The obtained enzymolysis solution was centrifuged at 8000 rpm for 10 minutes, and the obtained supernatant was the clam enzymolysis solution;

[0034] Separation and purification of antioxidant peptides: The enzymatic solution was separated by Sephadex G-25 gel chromatography, using pure water as the eluent, detected at 280nm, the flow rate was 3mL / min, and the antioxidant activity of each component was detected. The RPTE2 fraction with the highest activity was collected, freeze-dried and made into an 80 mg / mL solution with deionized water, and further separated by Sephadex G-15 gel chr...

Embodiment 3

[0036] (1) Enzymatic hydrolysis preparation of antioxidant peptides: After washing the live Filipino clams with water, remove the shells, take the meat, and make a pulp. Add a phosphate buffer solution with a pH of 7.5 and a concentration of 0.01mol / L according to the ratio of solid to liquid 1:6, add trypsin 6600U / g at 45°C for 4 hours, and inactivate the enzyme at 97°C for 5 minutes, and the obtained The enzymolyzate was centrifuged at 8000rpm for 10min, and the obtained supernatant was the clam enzymolyzate;

[0037] Separation and purification of antioxidant peptides: the enzymatic solution was separated by Sephadex G-25 gel chromatography, using pure water as the eluent, detected at 280nm, the flow rate was 1.5mL / min, and the antioxidant activity of each component was detected , collect the RPTE2 fraction with the highest activity, freeze-dry it, make a 65mg / mL solution with deionized water, and further separate it by Sephadex G-15 gel chromatography, use pure water as th...

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Abstract

The present invention relates to a Ruditapes philippinarum anti-oxidation peptide and a preparation method thereof, wherein the amino acid sequence of the Ruditapes philippinarum anti-oxidation peptide is Leu-Phe-Lys-Lys-Asn-Leu-Leu-Thr-Leu, and enzymolysis preparation of the anti-oxidation peptide, separation purification of the anti-oxidation peptide, and reverse chromatography-Triple TOF analysis and retrieval of the anti-oxidation peptide are performed to obtain the anti-oxidation peptide. According to the present invention, the used raw material is the Ruditapes philippinarum meat, such that the sources are wide, the price is low, and the value rising is considerable after the Ruditapes philippinarum meat is adopted as the raw material and is developed into the anti-oxidation peptide; and the anti-oxidation peptide has the strong activity, can be adopted as the food additive, and can further be used as the functional ingredient for health products, cosmetics and skin care products.

Description

technical field [0001] The invention relates to a Philippine clam antioxidant peptide and a preparation method thereof, belonging to the field of food biotechnology. Background technique [0002] Under normal metabolic pathways, the production and elimination of free radicals in the body are in a dynamic equilibrium state, but with age or when the body is in an adverse environment, the balance of free radicals in the body will be broken, and the balance Once broken, excess free radicals that cannot be eliminated by the body remain in the body. Excessive free radicals can cause the aging of the body, and even lead to the emergence of various diseases. [0003] Free radical scavengers are also called antioxidants, which can scavenge free radicals and reduce or eliminate the damage of free radicals to the body. Synthetic antioxidants such as propyl gallate have been proven to be toxic and carcinogenic to humans. Therefore, searching for safe and efficient natural antioxidant...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/16C12P21/06G01N30/02
Inventor 程文健刘文钊张惠婷赵卉双钟机陈丽娇
Owner FUJIAN AGRI & FORESTRY UNIV
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