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Biocomposite material, preparing method and use thereof

A composite material and biological technology, applied in medical science, prosthesis, etc., can solve the problems of high cost, short residence time of necrotic area, difficulty in maintaining activity, etc., and achieve the effect of promoting bone growth

Inactive Publication Date: 2015-10-21
THE CHINESE UNIVERSITY OF HONG KONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Allogeneic bone grafting avoids the disadvantages of autologous bone, but there are potential infection opportunities and rejection reactions
Bone morphogenetic protein (BMP) is a growth and differentiation factor that can be used to promote local bone growth, but this protein degrades quickly and requires a large dose to maintain its effect, resulting in high cost; its physical and chemical properties are unstable, unlike other Difficulty maintaining activity when vector is mixed; short residence time in necrotic areas
Gene therapy is only in the stage of animal experiments due to safety issues, and it cannot be applied clinically at present

Method used

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  • Biocomposite material, preparing method and use thereof
  • Biocomposite material, preparing method and use thereof
  • Biocomposite material, preparing method and use thereof

Examples

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preparation example Construction

[0035] In a third aspect, a method for preparing a biocomposite according to the present invention is provided, the method comprising the steps of:

[0036] (1) Polylactic acid / glycolic acid copolymer and tricalcium phosphate are dissolved in the first organic solvent;

[0037] (2) dissolving icariin in the same or different second organic solvent as step (1);

[0038] (3) adding the solution obtained in step (2) to the solution obtained in step (1), and mixing to obtain a slurry containing PLGA, TCP and icariin; and

[0039] (4) Using rapid prototyping technology to shape the above slurry to obtain the biocomposite material.

[0040] In an embodiment of the present invention, the first organic solvent may be dioxane, dichloromethane, chloroform, dimethylsulfoxide and the like. In a preferred embodiment, the first organic solvent is dioxane.

[0041] In an embodiment of the present invention, the second organic solvent may be dioxane, dichloromethane, chloroform, dimethyl sul...

Embodiment 1

[0053] Preparation of Biocomposites Composed of PLGA, TCP and Icaritin

[0054] 1. Materials and Equipment

[0055] PLGA was purchased from Shandong Medical Device Research Institute, the molecular weight of PLGA was 100,000, and the molar ratio of lactic acid to glycolic acid was 70:30;

[0056] TCP was purchased from Beijing Modern Oriental Fine Chemical Products Co., Ltd.;

[0057] Icaritin was provided by Shenzhen Center of Traditional Chinese Medicine and Natural Medicine; and

[0058] The biomaterial rapid prototyping machine was developed by the Department of Mechanical Engineering, Tsinghua University, and the model is CLRF-2000-II.

[0059] 2. The preparation process of the biocomposite (refer to figure 2 preparation roadmap)

[0060] (1) Add 5.2g PLGA and 1.3g TCP to 40ml dioxane, dissolve and stir for 24 hours;

[0061] (2) 1.6mg of icariin was added to 10ml of dioxane for dissolution;

[0062] (3) Take 1ml of icariin dissolved in dioxane in step (2) and add ...

Embodiment 2

[0068] Slow release of icariin from biocomposites

[0069] Take 150 mg of the biocomposite prepared in Example 1 and add it into 50 ml of phosphate buffered saline (PBS, pH 7.4). Place the above mixture in a shaking water bath at 120 rpm at 37 °C. On days 1, 2, 7, and 14, 5 ml of liquid was taken out and replenished with an equal volume of fresh PBS. The withdrawn liquid was extracted three times with an equal volume of ethyl acetate and then dried with nitrogen. The dried product obtained was dissolved in 100 µl of methanol, and was fully dissolved by shaking. The concentration of icariin was determined by high performance liquid chromatography (HPLC) at a detection wavelength of 270nm.

[0070] Such as image 3 Shown, the release cumulative amount of icariin on the 1st day is 13.5%, the release cumulative amount on the 2nd day is 20.5%, the percentage cumulative release amount on the 7th day is 30.0%; The cumulative released amount of toxin was 39.5%. These results ind...

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Abstract

The present invention relates to a biocomposite material which comprises biodegradable polylactic acid / glycolic acid copolymer, tricalcium phosphate and effective amount of icaritin. The invention also relates to a preparing method of the biocomposite material and an artificial bone support prepared by the biocomposite material. Furthermore, the invention also relates to the use of the biocomposite material in treating tissue defect, particularly in preparing bone defect.

Description

technical field [0001] The present invention relates to biocomposite materials, more specifically, to a high molecular polymer composite material combined with bioactive molecules. The invention also relates to a method for the preparation of the biocomposite material and its use. Background technique [0002] Bone defect is a difficult point in orthopedic surgery. Factors leading to bone defects include trauma, loose bone fractures, tumors, and osteonecrosis. In particular, the incidence of bone defects caused by osteonecrosis is high, which poses a great threat to human health and quality of life. [0003] Incomplete repair occurs in the early stages of osteonecrosis, and the reasons for incomplete repair include decreased activity of bone marrow stem cell pool, apoptosis of osteocytes and degradation of cancellous bone matrix. This incomplete repair should therefore be prevented by providing sufficient osteogenic precursor cells and / or increasing the osteogenic capacit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/12A61L27/18A61L27/54
Inventor 秦岭王小红谢鑫荟王新峦张戈姚新生梁国穗冯国培严永年彭江陈启明郑振耀熊良俭
Owner THE CHINESE UNIVERSITY OF HONG KONG
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