Preparation method for gold labeled immucochromatographic test strip jointly marked through colloidal gold and latex microsphere

A technology of immunochromatographic test paper and latex microspheres, which is applied in analytical materials, biological testing, biological material analysis, etc., can solve problems such as unfavorable inspection and promotion, and achieve improved detection sensitivity and detection range, good dispersion, and strong practical value. and realistic effects

Inactive Publication Date: 2015-10-21
宋晓峰 +1
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Problems solved by technology

However, because the detection process requires a large-scale instrument such as a fully autom

Method used

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Embodiment Construction

[0017] Embodiments of the present invention will be described in detail below. The preparation method of the gold-labeled immunochromatography test strip of a kind of colloidal gold and latex microsphere joint label of the present invention comprises the following steps:

[0018] (1) Utilize trisodium citrate to reduce chloroauric acid to prepare a colloidal gold solution, and the prepared colloidal gold particle size is in the range of 30nm~40nm;

[0019] (2) Label protein molecules with colloidal gold solution, because the adsorption of protein mainly depends on the pH value. Under the conditions close to the isoelectric point of protein or partial alkalinity, the colloidal gold solution and protein molecules are easy to form a strong bond. , if the pH value of colloidal gold is lower than the isoelectric point of protein, it will aggregate and lose its binding ability. In addition, the size of colloidal gold particles, ionic strength, and molecular weight of protein all aff...

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Abstract

The invention discloses a preparation method for a gold labeled immucochromatographic test strip jointly marked through colloidal gold and latex microsphere. The preparation method comprises the following steps that 1 chloroauric acid is reduced through trisodium citrate to prepare colloidal gold solution; 2 the colloidal gold solution is used for marking protein molecules; 3 50 nm red latex microsphere is prepared or bought directly; 4 the protein molecule is marked by the latex microsphere; 5 quality testing is conducted on the marking colloidal gold solution and latex marking solution which are obtained through the above steps; 6 a nitrocellulose membrane is pasted on a PVC plastic bottom plate; 7 a sample pad, a combining pad, a water-absorbing pad, handle paper and Max glue are assembled on the PVC plastic bottom plate; 8 quality testing is conducted on the prepared gold labeled test strip according to the quality standard of the product. The preparation method for the gold labeled immucochromatographic test strip jointly marked through the colloidal gold and the latex microsphere has the advantages that the detecting sensitivity and detecting range of the gold labeled immucochromatographic test strip are improved, and the application area and quantitative application of colloidal gold immunologic diagnosis are enlarged in this way.

Description

technical field [0001] The invention relates to a method for preparing a gold-labeled immunochromatographic test strip jointly labeled with colloidal gold and latex microspheres, and belongs to the technical field of in vitro diagnostic reagents in the field of biomedicine. Background technique [0002] At present, the research on the traditional colloidal gold labeling technology began in the early 1960s. Colloidal gold is polymerized by chloroauric acid (HAuCl4) under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, and tannic acid. The size of gold particles, and due to electrostatic interaction into a stable colloidal state, called colloidal gold. Colloidal gold is negatively charged in a weak alkaline environment and can form a firm bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological characteristics of the protein. Because the color of colloidal gold is r...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/68G01N33/531G01N33/58
CPCG01N33/558G01N33/531G01N33/585G01N33/6803G01N2333/4737
Inventor 宋晓峰
Owner 宋晓峰
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