Application of Cordyceps sinensis in the prevention and treatment of chronic vascular dementia
A technology for vascular dementia and Cordyceps sinensis, applied in the field of medicine, can solve the problems of high price, long growth period, difficult collection and the like
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Embodiment 1
[0044] Example 1 The effect of preventive administration of Bailing Capsules on the novel object recognition test of rUCCAO mice
[0045] 1. Set up animal groups: male ICR mice (8-10 weeks, 22-25 g, 10 mice in each group), were randomly divided into groups, and acclimatized to the environment for one week before the start of the experiment. Grouped as Table 1.1
[0046] Table 1.1 Animals and groups required in Example 1
[0047]
[0048] 2. Administration and dosage: The content powder of Bailing Capsules is weighed and dissolved in 1% CMC-Na (sodium carboxymethylcellulose) solution to make a suspension, and the animals are administered orally by gavage, the dose is : 0.2g / kg body weight, 1.0g / kg body weight, once a day. The prophylaxis group was administered from 7 days before modeling to the end of the day of modeling.
[0049] 3. Surgical process: After the animal is anesthetized, the surface is disinfected, the skin on the top of the skull is cut, and the Doppler cer...
Embodiment 2
[0052] Example 2 The effect of preventive administration of Bailing Capsules on the Morris water maze test in rUCCAO mice
[0053] Animal grouping, administration and dosage, and operation process are the same as in Example 1. The water maze consists of a pool with a diameter of 150cm, a height of 50cm, and a water temperature of 20°C, a camera and an analysis system. The water depth of the pool is 30cm, and the circular water surface is divided into 4 quadrants by two imaginary vertical lines passing through the center of the circle. Place an escape platform with a diameter of 10 cm at a distance of 1.5 cm from the water surface in the middle water area of one of the quadrants. Add carbon ink to the water and mix well to make it black, so as to prevent mice from directly observing the platform. Randomly put the mice into the water from four different quadrants of 1, 2, 3, and 4 in the pool. When placed, the mice face the platform with their backs. After entering the water...
Embodiment 3
[0055] Example 3 Effect of preventive administration of Bailing Capsules on white matter damage in rUCCAO mice
[0056] Animal grouping, administration and dosage, and operation process are the same as in Example 1. After mice were anesthetized with chloral hydrate, brain tissue was separated after perfusion with 4% paraformaldehyde (0.1M phosphate buffer, pH=7.4), immersed in 4% paraformaldehyde at 4°C for 24 hours in the dark, and then immersed in 30% paraformaldehyde Sucrose solution for 3 days, sliced with a cryostat. After the frozen sections were air-dried, put them in absolute alcohol for 5-10 minutes, then put the sections into sealed Luxol fast blue (LFB) staining bottles, and put them in a water bath at 56°C overnight. The next day, the slides were taken out and brought back to room temperature, and the slices were placed in 95% ethanol for 3 minutes, 70% ethanol for 3 minutes, double distilled water for 3 minutes, 0.05% lithium carbonate aqueous solution for 5-10...
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