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TuVIPP1 protein and encoding gene and application of TuVIPP1 protein

A protein and encoding technology, applied in the field of TuVIPP1 protein and its encoding gene and application, can solve the problems of complex genome, difficult work, and long functional cycle of crops

Active Publication Date: 2015-10-28
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fundamental reason is that the genome of crops is complex, and it is difficult to study the functions of important genes in a long cycle

Method used

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  • TuVIPP1 protein and encoding gene and application of TuVIPP1 protein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1, Cloning and sequencing analysis of TuVIPP1

[0054] Urartu wheat (Triticum urartu) variety G1812 (Draft genome of the wheat A-genome progenitor Triticum urartu. Hong-Qing Ling etc., Nature, volume 496, phase 7443, page number 87-90.) The 10-day seedlings get 100 mg of leaves, Use the EasyPure Plant RNA kit (Code#ER301-01) and TransScript One-Step gDNA Removaland cDNA Synthesis SuperMix (Code#AT311-02) from Quanshijin Company to extract total RNA according to the operating instructions, and obtain the full-length TuVIPP1 cDNA by RT-PCR, as follows :

[0055] 1. Reverse transcription

[0056] Using the extracted total RNA as a template, the following reverse transcription system was used to obtain cDNA.

[0057] The reverse transcription system is:

[0058] Total RNA 5ul

[0059] Oligo-dT 1ul

[0060]RNase-free Water 2ul

[0061] Mix the above solution with a centrifuge tube, 65°C for 5 minutes, ice bath for 2 minutes, continue to add the following soluti...

Embodiment 2

[0080] Embodiment 2, the application of TuVIPP1 in improving plant photosynthesis

[0081] 1. Identification of Arabidopsis Atvipp1 T-DNA insertion mutant Atvipp1(+ / -)

[0082] The Arabidopsis Atvipp1 T-DNA insertion mutant Atvipp1 (+ / -) numbered Sail_5_F07 was purchased from NASC (The European Arabidopsis Stock Centre, only the VIPP1 gene was deleted, and the rest of the genes were consistent with the wild type). This mutant has a T-DNA fragment inserted between exons 3 and 4, which completely inhibits the expression of VIPP1 gene and produces a semi-lethal phenotype. In order to achieve the reliability and authenticity of the experiment, the purchased Arabidopsis mutants need to be further identified at the molecular level and the macroscopic (phenotype) level.

[0083] 1. Phenotype identification of mutant Atvipp1(+ / -)

[0084] Appropriate amounts of dried Arabidopsis mutant Atvipp1(+ / -) and wild-type Arabidopsis col-0 were purchased from NASC (The European Arabidopsis St...

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Abstract

The invention discloses TuVIPP1 protein and an encoding gene and application of the TuVIPP1 protein. The protein is the protein in (1) or (2). The protein in (1) is composed of amino acid residues shown in the sequence 2 in a sequence table; the protein in (2) has the same function, is derived from (1) and is obtained after an amino acid sequence residue sequence in the sequence 2 in the sequence table is subjected to replacing and / or deleting and / or adding of one or more amino acid residues. Experiment results show that the TuVIPP1 gene is cloned in diploid Urartu wheat, the key function of the gene in inner capsule generation is verified by transgenic complementary of arabidopsis thaliana Atvipp1T-DNA insertion deletion mutant, plant photosynthesis can be promoted, and albino plants are greened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a TuVIPP1 protein and its coding gene and application. Background technique [0002] VIPP (vesicle-inducing protein in plastids, plastid vesicle induction protein) is a chloroplast-localized protein, which is reported to be distributed on the inner membrane, stroma and endosome membrane of Chlamydomonas and Arabidopsis chloroplasts. The vipp gene Deletion mutants have serious defects in endocapsule structure and cannot be used for photoautotrophy and other phenotypes. In major crops, the function of VIPP gene has not been reported yet. [0003] In recent years, using the model plant (Arabidopsis thaliana) system as a means to study the research strategies of important genes in crops such as soybean and wheat has been widely used by scientists. The fundamental reason is that the crop genome is complex, and it is difficult to study the functions of its important genes for a long peri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C12N15/82A01H1/04A01H5/00
CPCA01H1/04C07K14/415C12N15/8261C12N15/8269
Inventor 高飞张文娟刘翠敏
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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