Method for enzyme extraction of echinacea polysaccharide
An extraction method and technology of Echinacea, applied in the directions of fermentation, drug combination, allergic diseases, etc., can solve the problems of low extraction rate and long extraction time of Echinacea polysaccharide, and achieve the effects of high extraction rate, short extraction time and good solubility
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Embodiment 1
[0031] The extraction method includes the following steps:
[0032] ① Medicinal material pretreatment: crush the aboveground part of Echinacea purpurea through a 60-mesh sieve; add a degreasing solvent, after degreasing, evaporate petroleum ether to dryness at 60°C, and use the treated medicinal material for later use;
[0033] ②Extraction: Weigh 100g of the medicinal material obtained in ①, add 1500g of water, adjust the pH to 3, preheat to 50°C, add 5g of pectinase, enzymatic hydrolysis time 60min, inactivate enzyme at 80°C for 10min, continue at 80°C Stir and keep warm for 1-h;
[0034] ③ Concentration: centrifuge, and concentrate the supernatant to a concentrated solution with a crude drug amount of 1:0.9 by rotary steaming and reducing pressure;
[0035] ④ Alcohol precipitation: add 3 times of absolute ethanol to the concentrated solution, and let it stand at 4°C for 24 hours;
[0036] ⑤ Washing: Suction filter the alcohol precipitation solution obtained in ④, and wash ...
Embodiment 2
[0039] The extraction method includes the following steps:
[0040] ① Medicinal material pretreatment: crush the aboveground part of Echinacea purpurea through a 40-mesh sieve; add petroleum ether, after degreasing, evaporate the petroleum ether to dryness at 60°C, and use the processed medicinal material for later use;
[0041] ②Extraction: Weigh 100g of the medicinal material obtained in ①, add 1500g of water, adjust the pH to 2, preheat to 40°C, add 4g of pectinase, enzymatic hydrolysis time 50min, inactivate the enzyme at 80°C for 10min, continue at 80°C Stir and keep warm for 1h;
[0042] ③ Concentration: centrifuge, and concentrate the supernatant to a concentrated solution with a crude drug amount of 1:0.9 by rotary steaming and reducing pressure;
[0043] ④ Alcohol precipitation: add 3 times of absolute ethanol to the concentrated solution, and let it stand at 0°C for 24 hours;
[0044] ⑤ Washing: Suction filter the alcohol precipitation solution obtained in ④, and w...
Embodiment 3
[0047] The extraction method includes the following steps:
[0048] ① Medicinal material pretreatment: crush the aboveground part of Echinacea purpurea through a 60-mesh sieve; add petroleum ether degreasing solvent, after degreasing, evaporate the petroleum ether to dryness at 60°C, and use the processed medicinal material for later use;
[0049] ②Extraction: Weigh 100g of the medicinal material obtained in ①, add 2500g of water, adjust the pH to 4, preheat to 60°C, add 6g of pectinase, enzymatic hydrolysis time 70min, inactivate the enzyme at 100°C for 20min, continue at 100°C Stir and keep warm for 2h;
[0050] ③ Concentration: Centrifuge, and concentrate the supernatant to a concentrated solution with a crude drug amount of 1:1.5 by rotary steaming and reducing pressure;
[0051] ④ Alcohol precipitation: add 4 times of absolute ethanol to the concentrated solution, and let stand at 4°C for 48 hours;
[0052] ⑤ Washing: Suction filter the alcohol precipitation solution ob...
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