Application of composite microspheres in the preparation of medicines for treating bone defects
A technology for compounding microspheres and bone defects, which is applied in the application field of medicine to achieve the effects of convenient use, improved curative effect and less toxic and side effects
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[0025] The preparation method of composite microsphere is as follows:
[0026] Step 1) Prepare large balls, prepare small balls by double emulsion method, large balls are loaded with anti-inflammatory drugs by surface adsorption, and small balls are loaded with pro-angiogenic growth factors through double emulsification; then use polyetherimide (Polyetherimide) on the surface of small balls , PEI) modification, the concentration of PEI is 0.01-0.5%, and the pellets are stirred in the PEI solution for 7-17 hours.
[0027] Step 2) Mixing and assembling the modified small balls and large balls, the mixing time of small balls and large balls is 2-6 hours, and the composite microspheres of large balls loaded with small balls are obtained.
Embodiment 1
[0029] This embodiment adopts the following technical scheme: preparing calcium silicate hollow microspheres with DEX adsorbed on the surface as large composite microspheres. Weigh 5-15mg of porous PLGA microspheres loaded with VEGF as the pellets of composite microspheres, add 5ml of 0.01-0.5% PEI solution, shake on a shaking table at a speed of 120rpm for 4h, mix evenly, and make the surface of the PLGA microspheres Uniform adsorption of PEI. Then the mixture was centrifuged with secondary water, the centrifugation speed was controlled at 7000rpm, the centrifugation time was 10min, and the centrifugation was repeated 3 times to remove the PEI that was not firmly adsorbed. Move the washed PLGA microspheres into a centrifuge tube, add about 1ml of secondary water, add 50-120mg of calcium silicate hollow microspheres adsorbed with DEX, then shake on a shaker for 4 hours, freeze-dry after centrifugation, and then The double drug-loaded microspheres can be prepared by grafting P...
Embodiment 2
[0031]Prepare large spheres of hydroxyapatite hollow microsphere composite microspheres with DEX adsorbed on the surface. Weigh 5-15mg of porous PLGA microspheres loaded with VEGF as the pellets of composite microspheres, add 5ml of 0.01-0.5% PEI solution, shake on a shaking table at a speed of 120rpm for 4h, mix evenly, and make the surface of the PLGA microspheres Uniform adsorption of PEI. Then the mixture was centrifuged with secondary water, the centrifugation speed was controlled at 7000rpm, the centrifugation time was 10min, and the centrifugation was repeated 3 times to remove the PEI that was not firmly adsorbed. Transfer the washed PLGA microspheres into a centrifuge tube, add about 1ml of secondary water and 50-120mg of hydroxyapatite hollow microspheres, then shake on a shaker for 4 hours, centrifuge and then freeze-dry to graft PLGA into On the surface of hydroxyapatite microspheres, double drug-loaded microspheres can be prepared. The microspheres can be direct...
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