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Method for extracting, separating and purifying psoralen and isopsoralen from fructus psoraleae

A technology for separation and purification of isopsoralen and isopsoralen, which is applied in the field of extraction of psoralen and isopsoralen, can solve the problems of cumbersome operation process, difficult selection of solvent system, low recovery rate, etc., and achieve low comprehensive cost, The process is green and environmentally friendly, and the effect of simple extraction

Inactive Publication Date: 2015-11-04
XUZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to literature reports, the methods for separating and purifying psoralen coumarin mainly include repeated silica gel column chromatography and high-speed countercurrent chromatography. Strong organic solvent; the choice of solvent system in high-speed countercurrent chromatography is difficult and lacks theoretical guidance

Method used

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  • Method for extracting, separating and purifying psoralen and isopsoralen from fructus psoraleae
  • Method for extracting, separating and purifying psoralen and isopsoralen from fructus psoraleae
  • Method for extracting, separating and purifying psoralen and isopsoralen from fructus psoraleae

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Embodiment

[0026] A method for extracting, separating and purifying psoralen and isopsoralen from psoralen, the steps of which are:

[0027] (1) Extraction of psoralen coumarin: pulverize the psoralen medicinal material, heat and extract 3 times with 8 times the amount of 70% ethanol-water solution, each time for 1.5h, filter, combine the extracts, concentrate under reduced pressure to obtain crude Extract.

[0028] (2) Separation and analysis of psoralen coumarin: use analytical high performance liquid chromatography to separate and analyze the composition of the crude extract, and the chromatographic column is C 18 Column (250×4.6mm I.D., 5μm), the mobile phase is methanol-water (45:55, V / V); the flow rate is 1.0mL / min, the detection wavelength is 254nm, and the column temperature is room temperature.

[0029] (3) Separation and purification of psoralen coumarin: use a semi-preparative high-performance liquid chromatograph to separate and purify the components in the crude extract, an...

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Abstract

The invention discloses a method for extracting, separating and purifying psoralen and isopsoralen from fructus psoraleae, relating to the technical field of extraction of psoralen and isopsoralen. The method comprises the following steps: heating an organic solvent under reflux to extract a coumarin component; carrying out isolation analysis on the composition of the extract by using an analytic-type high performance liquid chromatograph; carrying out separation and purification on the components in the extract by using a semipreparative high performance liquid chromatograph to obtain 2 compounds; and finally, determining the purity of the compounds by high performance liquid chromatography. The method can efficiently, quickly and simply extract, separate and purify the psoralen and isopsoralen from fructus psoraleae.

Description

technical field [0001] The invention relates to the technical field of extracting psoralen and isopsoralen, in particular to a method for extracting, separating and purifying psoralen and isopsoralen from psoralen. Background technique [0002] Psoralen is the dried and mature fruit of the leguminous plant Psoralea corylifolia L., which has the functions of nourishing the kidney and strengthening yang, strengthening the body, relieving qi and stopping diarrhea, and gynecological hemostasis. , Deficiency, cold, cough and asthma, and diarrhea before dawn. Pharmacological studies have shown that psoraleae can dilate blood vessels, enhance myocardial contractility, and improve the body's non-specific immune function. It is unique in antibacterial, antiviral, antitumor, and antiaging. disease, vitiligo, alopecia areata and osteoporosis. Psoralen mainly contains coumarin, flavonoids and other components. Psoralen and isopsoralen are representatives of coumarin components, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04
CPCC07D493/04
Inventor 杨方秀孙世安汤道权陆益红栗政董露露
Owner XUZHOU MEDICAL COLLEGE