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Nogo-A receptor binding peptide as well as derivative and application thereof

A receptor binding and derivative technology, applied in the field of biomedicine, can solve the problems of nerve function recovery of patients, achieve huge social and economic benefits, promote recovery, and promote nerve regeneration.

Active Publication Date: 2015-11-18
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no effective drug for this type of disease that can restore the patient's neurological function

Method used

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  • Nogo-A receptor binding peptide as well as derivative and application thereof
  • Nogo-A receptor binding peptide as well as derivative and application thereof
  • Nogo-A receptor binding peptide as well as derivative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Organic Synthesis and Detection of Nogo-A Receptor Binding Peptides

[0048] Organically synthesized No. 2 polypeptide (His-Ile-Tyr-Thr-Ala-Leu-Val) and No. 5 polypeptide (Gly-Phe-Ala-Thr-Ile-Thr-Gly) (synthesized by Shanghai Qiangyao Biological Company), called Take 1g of KP resin and place it in a reactor, add DMF to swell for 30min, filter out DMF, dissolve Fmoc amino acid, 0.4mmol of HBTU, HOBT and 0.6mmol of NMM in an appropriate amount of DMF, add it to the reactor, and set it at 40°C Shake for 2 hours, filter the reaction solution, wash the resin twice with methanol and DMF alternately, and wash once with anhydrous ether; elute the Fmoc group with 20% piperidine / DMF solution by volume fraction; react for 10 minutes, filter the reaction solution methanol, Wash the resin twice with DMF alternately; carry out the coupling of the next amino acid residue, so that the first amino acid at the C-terminal is extended to the N-terminal until the short peptide is ...

Embodiment 2

[0050] Example 2 ELISA detection of phage display: NgR specificity, specificity binding positive monoclonal

[0051] A 96-well ELISA plate (Corning, Inc.) was coated with sNgR protein diluted in PBS at a concentration of 2.5 μg / mL (R&D Company, USA), 150 μL per well, and the wells not coated with sNgR were used as a control. Shake slowly overnight at 4°C, pour off the coating solution, add a blocking solution with a mass fraction of 1% BSA diluted in PBS, and block for 1 hour; add the monoclonal 1×10 10100 μL of pfu / mL phage (NewEngland Biolabs, Beverly, MA), combined with slow shaking at room temperature for 1 h; then washed 6 times with 0.1% PBST, and added 100 μL of HRP-anti-M13 antibody (NEB Company) (diluted 1:5000) , incubate at 37°C for 40 min, wash with PBST for 6 times, add 50 μL of the display substrate TMB (Beiyuntian Company) for 10 min, 1MH 2 SO 4 Stop the color development, and detect the OD value with a microplate reader at 450nm. According to the results of ...

Embodiment 3

[0052] Example 3 Confocal binding of Nogo-A receptor-binding peptides to NgR on the cell membrane surface of PC-12 and CGCs cells with high NgR expression, and colocalization of Nogo-A receptor-binding peptides with NgR on the surface of PC-12 cells

[0053] Confocal plates were inoculated with PC-12 and CGCs cells, among which PC12 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (ATCCCRL-1721), and CGCs cells were obtained from Sprague-Dawley (SD) suckling mice (SPF grade, Guangdong Province, 6-8 days after birth) Center (SCXK Guangdong 2013-0002)); 10,000 cells per dish, cultured for 24 hours, washed three times with PBS, fixed with paraformaldehyde for 15 minutes, washed three times with PBS, blocked with BSA for 1.5 hours, and then labeled with 1 μM FITC peptide No. 2 Or polypeptide No. 5 (the FITC markers of polypeptide No. 2 or No. 5 were prepared by Shanghai Qiangyao Biological Co., Ltd.), incubate at room temperature for 2 hours (co-localizati...

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Abstract

The invention belongs to the field of biological medicine, and particularly relates to a Nogo-A receptor binding peptide as well as a derivative and an application thereof. The amino acid residue sequence of the Nogo-A receptor binding peptide is HIYTALV or GFATITG. The derivative of the binding peptide is a product obtained through conventional modification of an amino terminal or a carboxyl terminal of a Nogo-A receptor binding peptide fragment on a Nogo-A receptor binding peptide amino acid side chain group or a product obtained by connecting a label used for peptide or protein detection or purification on the Nogo-A receptor binding peptide. The binding peptide and the derivative of the binding peptide can be bound with NgR in vitro, nerve regeneration is promoted by preventing binding of NgR and Nogo-A, nerve regeneration barriers caused by central nervous injury diseases can be treated, recovery of a central nervous function is promoted, and the binding peptide and the derivative can be widely applied in the medical and biological fields.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a Nogo-A receptor binding peptide, derivatives and applications thereof. Background technique [0002] With the prolongation of human life span, the problem of population aging has become increasingly prominent, and the incidence of some central nervous system (central neverous system, CNS) diseases is increasing, but effective treatment methods are lacking. The main reason is that the central nervous system is the headquarters of the human body for processing information, and its function depends on the neural network formed by a large number of basic structural unit neurons. Since the neuron itself cannot divide, once the neuron is damaged, the reconnection of the neural network can only be achieved through the regrowth of the neurite, and then the recovery of the function can be achieved, and the ability of the damaged neuron itself to regenerate is very weak, which leads...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K19/00A61K38/08A61P25/28A61P25/16A61P25/00A61P9/10
Inventor 肖飞孙中轻代小勇林熙武征苏志坚黄亚东罗焕敏
Owner JINAN UNIVERSITY
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