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Ultrasonic wave aided extraction method suitable for high-viscosity collagen

An ultrasonic-assisted extraction and collagen technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and animal/human proteins, etc., can solve the problems of attenuation, unsatisfactory ultrasonic-assisted extraction, and insufficient ultrasonic effect of materials. The effect of reducing extraction cost, preventing damage and improving extraction efficiency

Inactive Publication Date: 2015-11-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For materials with high viscosity, the attenuation of the ultrasonic treatment process is serious, which will lead to insufficient ultrasonic effect of the material, and the ultrasonic-assisted extraction effect is not ideal

Method used

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  • Ultrasonic wave aided extraction method suitable for high-viscosity collagen
  • Ultrasonic wave aided extraction method suitable for high-viscosity collagen
  • Ultrasonic wave aided extraction method suitable for high-viscosity collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Such as figure 1 As shown, this ultrasonic-assisted extraction method suitable for high-viscosity collagen is characterized in that it comprises the following steps:

[0034] 1) Soak the material to be extracted in the acidic solution to expand the material to be extracted, and then place the acidic solution soaked in the material to be extracted in the storage mixer 1 for stirring to form a uniform mixed solution;

[0035] 2) Send the mixed solution into the ultrasonic reactor 3 through the feed pump 2, and the ultrasonic reactor 3 performs pulsed ultrasonic treatment on the solution;

[0036] 3) Circulation pump 4 extracts the mixed solution that has been subjected to ultrasonic treatment from the bottom of ultrasonic reactor 3, and the mixed solution is pumped into heat exchanger 5 for cooling;

[0037] 4) After the cooled mixed solution comes out from the heat exchanger 5, it is sent back to the ultrasonic reactor 4 for further ultrasonic treatment;

[0038] 5) Re...

Embodiment 2

[0046] This ultrasonic-assisted extraction method suitable for high-viscosity collagen is the same as Example 1 except for the following technical features:

[0047] The parameters of the generator are set to 20kHz, 600W. Soak the material to be extracted in 0.5mol / L acetic acid solution, that is, the acidic solution is acetic acid, and the material to be extracted is fish skin, and the mass ratio of fish skin to acetic acid solution in the mixed solution is 1:20. The volume of the mixed liquid is 1.2L; the time for the reactor to perform the pulsed ultrasonic reaction is 10min to 30min; and the flow rate of the circulating pump to pump the mixed liquid is 150ml / min. The refrigerator makes the temperature of the cold side channel of the heat exchanger 0°C to ensure the mixing of the mixed liquid in the reaction vessel at 4°C. Then the Lowry method was used to measure the dissolution amount of collagen under different ultrasonic-assisted extraction time and the denaturation te...

Embodiment 3

[0052] This ultrasonic-assisted extraction method suitable for high-viscosity collagen is the same as Example 1 except for the following technical features:

[0053] The parameters of the generator are set to 20kHz, 900W. Soak the material to be extracted in 0.5mol / L acetic acid solution, that is, the acidic solution is acetic acid, and the material to be extracted is fish skin, and the mass ratio of fish skin to acetic acid solution in the mixed solution is 1:20. The volume of the mixed liquid is 1.8L; the time for the reactor to perform pulsed ultrasonic reaction is 30min; and the flow rate of the circulating pump to draw the mixed liquid is 200ml / min. The refrigerator makes the temperatures of the cold side passages of the heat exchanger 0°C, 10°C and 28°C respectively, so as to ensure that the mixed liquids in the reaction vessel are mixed at 4°C, 15°C and 30°C. Measure the solubility and viscosity of collagen after ultrasonic-assisted extraction, use CD (circular dichroi...

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Abstract

The invention discloses an ultrasonic wave aided extraction method suitable for high-viscosity collagen. The ultrasonic wave aided extraction method comprises the following steps that firstly, materials with extraction to be carried out is steeped in an acid solution to be expanded, then the acid solution with the materials with extraction to be carried out steeped is put into a material storage stirrer to carry out stirring, and a uniform mixing solution is formed; secondly, the mixing solution is sent into an ultrasonic reactor through a feeding pump, and pulse type ultrasonic processing is carried out on the solution through the ultrasonic reactor; thirdly, the mixing solution with the ultrasonic processing carried out is pumped out of the bottom of the ultrasonic reactor through a circulation pump, and the mixing solution is pumped into a heat exchanger to be cooled; fourthly, the cooled mixing liquid comes out of the heat exchanger and then is sent to the ultrasonic reactor to carry out ultrasonic processing further; fifthly, the operation in the third step and the operation in the fourth step are repeated, circulation is carried out in this way for many times, and the extraction of the collagen is completed. By means of the ultrasonic wave aided extraction method, the extraction efficiency of the collagen can be improved, and the biological activity of the collagen can be maintained.

Description

technical field [0001] The invention relates to an extraction technology for heat-sensitive, high-viscosity materials, in particular to an ultrasonic-assisted extraction method suitable for high-viscosity collagen. Background technique [0002] Ultrasonic refers to the frequency of 2 × 10 4 ~1×10 7 Hz sound waves can produce physical effects such as cavitation, micro jets, and thermal effects. Cavitation refers to the physical and chemical effects produced during the formation, growth, oscillation, and collapse of ultrasonic cavitation bubbles; the microjet action is mainly the strong impact of cavitation bubbles when they collapse; the thermal effect is the medium Mechanical vibrations generated by absorbing ultrasonic waves convert ultrasonic energy into thermal energy. The cavitation effect of ultrasound can destroy the cell membrane and cell wall of natural substances, which can not only increase the speed of the solvent entering the cell, but also promote the rapid d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14
CPCC07K14/78
Inventor 朱志伟梁健华孙大文王启军曾新安
Owner SOUTH CHINA UNIV OF TECH
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