Method for fixing flocculated and settled lipid yeast through microbial flocculant cooperating with activated carbon

A microbial flocculant and oil yeast technology, applied in the field of microorganisms, can solve the problems of complex process and high cost, and achieve the effects of high-efficiency flocculation and sedimentation, simple operation and increasing particle size.

Inactive Publication Date: 2015-11-18
NORTHEAST DIANLI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the main bottleneck in the preparation of microbial oils at present is the complex process and high cost of separating and obtaining oil from the fermentation broth after oil-producing microorganisms accumulate oil in industrial production.

Method used

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  • Method for fixing flocculated and settled lipid yeast through microbial flocculant cooperating with activated carbon
  • Method for fixing flocculated and settled lipid yeast through microbial flocculant cooperating with activated carbon
  • Method for fixing flocculated and settled lipid yeast through microbial flocculant cooperating with activated carbon

Examples

Experimental program
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Effect test

preparation example Construction

[0025] (3) Preparation of Starley's Lipoyeast Culture Fluid

[0026] Inoculate Lipoyces studii with the bacterial strain number CGMCCNO: 2.1560 stored on the slant into a sterile seed medium, and the dosage of each component in the seed culture of Lipostaria studii is: glucose; (NH 4 ) 2 SO 4 ; Yeast powder; KH 2 PO 4 ;MgSO 4 ·7H 2 O; pH=6.0; cultivated on a shaker at 22°C at 150rpm for 1 day to obtain a cultured L. starii suspension, and stored the obtained suspension in a refrigerator at 4°C for future use;

[0027] (4) The immobilization of activated carbon to the strain No. CGMCCNO: 2.1560 Lipoyeast yeast

[0028] Take the suspension of L. starii obtained in step (3) and put it into a sterile nitrogen-limited medium. The dosage of each component in the nitrogen-limited medium is: glucose; (NH 4 ) 2 SO 4 ; Yeast powder; KH 2 PO 4 ;MgSO 4 ·7H 2 O; pH=6.5; take the activated carbon obtained in step (1), add it to the above-mentioned nitrogen-limited medium that ...

Embodiment 1

[0031] (1) Treatment of activated carbon

[0032] Take 20g of activated carbon with a specification of 300 mesh and dry it at 200°C for 2 hours; after cooling to room temperature, weigh the activated carbon and water according to the volume ratio of activated carbon and water as 1:3; Sulfuric acid; fully stirred for 2 hours at a water bath temperature of 80°C; then suction filtered, and the activated carbon was washed several times with desalted water until the pH value of the filtrate was neutral; the filtered activated carbon was dried at 200°C; the activated carbon was taken out and placed in Baked in a muffle furnace at 300°C for 2.5 hours and then placed in a desiccator for later use;

[0033] (2) Preparation of microbial flocculant by Aspergillus sojae

[0034] Inoculate Aspergillus sojae (Aspergillus sojae) stored on the slant with the strain number CGMCCNO: 3.5231 into 100 mL of sterile fermentation medium. The dosage of each component in the Aspergillus sojae ferment...

Embodiment 2

[0045] Including the same steps (1), (2) and (3) as in Example 1, the difference steps are:

[0046] (4) The immobilization of activated carbon to the strain No. CGMCCNO: 2.1560 Lipoyeast yeast

[0047] Take 10mL of the suspension of L. starii obtained in step (3) and put it into 100mL of sterile nitrogen-limited medium. The dosage of each component in the nitrogen-limited medium is: glucose 80.0g / L; (NH 4 ) 2 SO 4 1.0g / L; yeast powder 0.8g / L; KH 2 PO 4 1.0g / L; MgSO 4 ·7H 2 O1.0g / L; pH=6.5; take 4g of activated carbon obtained in step (1), add it to the above 100mL nitrogen-limited medium that has been inoculated with L. studley yeast, and cultivate it on a shaker at 22°C at 150rpm for 3 days. Obtain activated carbon-immobilized Liposaccharomyces starii suspension;

[0048] (5) Microbial flocculant flocculation and sedimentation activated carbon immobilized strain No. CGMCCNO: 2.1560 Lipoyeast sp.

[0049] Take 0.2mL of the microbial flocculant obtained in step (2), p...

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Abstract

The invention discloses a method for fixing flocculated and settled lipid yeast through microbial flocculant cooperating with activated carbon, belongs to the technical field of microorganisms, aims at utilizing characteristics of the activated carbon combined with microbial flocculation technology to flocculate and settle the lipid yeast and is is a method for fixing the flocculated and settled lipid yeast through the microbial flocculant cooperating with diatomite. The method includes the steps of treatment of the activated carbon, preparation of the microbial flocculant through aspergillus sojae, preparation of a Starkeyi lipid yeast culture solution, immobilization of Starkeyi lipid yeast through the activated carbon, and flocculation and settling of the Starkeyi lipid yeast immobilized by the activated carbon through the microbial flocculant. The immobilized Starkeyi lipid yeast is simple in operation and high in flocculation and settling rate which is maintained between 93.90% and 99.62%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. Background technique [0002] With the increasing shortage of petroleum resources, it is inevitable to develop renewable oil resources through multiple channels. Microbial oil, also known as single-cell oil, is a large amount of oil produced in bacteria by microorganisms such as yeast, mold, bacteria and algae under certain conditions, using carbohydrates, hydrocarbons and ordinary oil as carbon sources. Using microorganisms to produce oil, microbial cells proliferate quickly and the production cycle is short; the raw materials required for microbial growth are abundant and cheap; using microbial methods to produce oil is not limited by seasons and climate changes, and can be produced continuously on a large scale with low production costs. L. stariae can synthesize a large amount of oil in the cell, and is an efficient oil-producing microorganism. Lipoyeast Stari is commonly used in ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12R1/85
Inventor 于大禹聂刚乔楠张晓君魏群
Owner NORTHEAST DIANLI UNIVERSITY
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