Early identification method of plum radiation-induced mutative material

An identification method and variation technology, which is applied in the field of early identification of radiation breeding materials, can solve the problems of identification of variation materials and long growth cycle of fruit trees, and achieve the effects of low cost, convenient operation and saving breeding costs

Active Publication Date: 2015-11-18
POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

The growth cycle of fruit trees is long, and it is difficult to identify variant materials using traditional botanical morphological identification methods and pollen microscopic electron microscope scanning methods.

Method used

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  • Early identification method of plum radiation-induced mutative material
  • Early identification method of plum radiation-induced mutative material
  • Early identification method of plum radiation-induced mutative material

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Experimental program
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Embodiment 1

[0016] 1. Leaf collection: Collect the young leaves of March plum, radiation-delayed red meat mutants, and 5 radiation-induced March plum materials without significant variation in phenotype. The traits of March plum tree and its mutant fruit are as follows figure 1 .

[0017] 2. Extraction and detection of genomic DNA from plum leaves: Plant genomic DNA extraction kit (TIANGEN, Beijing) was used to extract DNA from March plum leaves. Take 5 μL of DNA solution and use 1% agarose gel electrophoresis to detect DNA purity. The electrophoresis buffer is 1×TAEBuffer. After electrophoresis for 20 minutes, the gel is stained with nucleic acid dye, and the gel image is collected with a gel imaging system. Finally, the DNA concentration of each material was diluted to 40ng / μL for use.

[0018] 3. PCR reaction system: commercial PCR mix 10 μL, primer 0.8 μL, template 1 μL, water 8.2 μL, the total volume is 20 μL. The PCR reaction program was as follows: pre-denaturation at 94°C for 5...

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Abstract

The invention relates to an early identification method of a plum radiation-induced mutative material and belongs to the technical field of breeding identification. The method comprises plum leaf acquisition, plum leaf genome DNA extraction and detection, PCR reaction system reaction, ISSR primer screening, plum radiation-induced mutative material ISSR analysis and gel image analysis. In radiation breeding, only a part of the material produces variation. A fruit tree has a long growth period so that the traditional botanical characteristic and form identification method and a pollen microscopic electron microscope scanning method are difficult to identify a mutative material. The early identification method can realize early identification of the plum radiation-induced mutative material, is convenient for operation and is simple and easy.

Description

technical field [0001] The invention belongs to the technical field of breeding identification, in particular to a method for early identification of radiation breeding materials. Background technique [0002] Radiation mutation breeding refers to the artificial use of a certain dose of physical radiation to irradiate plant materials to induce mutations, and artificial selection, identification, and cultivation of new varieties. Radiation mutagenesis is an effective way to cultivate new germplasm and new varieties of fruit trees. The radiation mutation breeding of fruit trees can increase the gene mutation frequency of fruit trees, shorten the breeding period, and obtain new germplasm that is difficult to obtain by conventional breeding in a short period of time. However, only some of the material was mutated during radiation breeding. The growth cycle of fruit trees is long, and it is difficult to identify mutant materials by traditional botanical morphological identifica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2531/113C12Q2525/151
Inventor 方智振叶新福周丹蓉潘少霖姜翠翠
Owner POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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