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Method for identifying or assisting in identifying shapes of potatoes and special primer pair for method

An auxiliary identification and potato technology, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of low screening efficiency, poor application effect, poor accuracy and stability, and achieve saving Manpower and material resources, accelerated breeding process, good stability

Active Publication Date: 2015-11-18
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few markers currently available for potato-shaped breeding, and even fewer markers for tetraploid potato-shaped breeding, and the currently-developed potato-shaped markers are less accurate and stable when applied to tuber-shaped screening , the screening efficiency is low, and the actual application effect is poor

Method used

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  • Method for identifying or assisting in identifying shapes of potatoes and special primer pair for method
  • Method for identifying or assisting in identifying shapes of potatoes and special primer pair for method
  • Method for identifying or assisting in identifying shapes of potatoes and special primer pair for method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, the method for identification or auxiliary identification of potato shape and the acquisition of molecular marker 1137-CAPSVI

[0043] 1. Preparation of test materials and phenotypic identification

[0044] 1. Test material

[0045] The test materials are as follows: long potato-shaped material 10618-01, round potato-shaped material 320-02 and 220 F1 potato-shaped materials obtained by crossing long potato-shaped material 10618-01 as the female parent and round potato-shaped material 320-02 as the male parent. Separate offspring.

[0046] 2. Genomic DNA extraction and quality testing

[0047]The potato leaves of the test materials in step 1 were collected, and the genomic DNA was extracted by the improved CTAB mini-method, and 2 μL of the genomic DNA was subjected to agarose gel electrophoresis for quality inspection. The electrophoresis detection results of some experimental materials are as follows: figure 1 Shown, indicating that the quality of the gen...

Embodiment 2

[0074] Example 2, Application of Molecular Marker 1137-CAPSVI in Diploid Potato and Tetraploid Potato Potato Shape Screening

[0075] 1. Application of molecular marker 1137-CAPSVI in diploid potato shape screening

[0076] 1. Phenotype identification of experimental materials and potato shape

[0077] The test materials were 213 diploid potatoes, which were derived from the F1 potato-shaped segregation offspring with the long potato-shaped material 10618-01 as the female parent and the round potato-shaped material 320-02 as the male parent. The phenotypic identification of 213 diploid potato shapes is all judged according to the judgment standard of 3 in step 1 of embodiment 1, and there are 133 round potato-shaped materials and 80 long potato-shaped materials in 213 diploid potato materials .

[0078] 2. Identification method and identification result

[0079] The genomic DNA of 213 diploid potatoes was used as a template, and the molecular marker 1137-CAPSVI was used as ...

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Abstract

The invention discloses a method for identifying or assisting in identifying shapes of potatoes and a special primer pair for the method. The method comprises the following steps: detecting whether the 805th deoxyribonucleotide of a target gene on a homologue of a potato to be tested is C or not, the 896th deoxyribonucleotide is T or not and the 1146th deoxyribonucleotide is A or not. The primer pair consists of DNA molecules shown in the sequence 1 of a sequence chart and DNA molecules shown in the sequence 2 of the sequence chart. Experiments show that when the primer pair is used for detecting the potato material, the fit goodness between the detection result and the phenotype identification result is 80% or above, and the fit goodness between the detection result and the round potato shape is 90% or above. The selection accuracy is high, the stability is good, the offspring can be selected in the seedling stage, influences from the plant growth stage and environmental conditions are prevented, the shape breeding process of potatoes can be effectively accelerated, and the breeding cost is lowered.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying or assisting in identifying potato shape and a pair of special primers. Background technique [0002] Potato is the third largest food crop in the world and the fourth largest food crop in my country. Because of its advantages of cold resistance, barren resistance, high and stable yield, wide adaptability, and comprehensive nutrition, it is widely planted around the world, which is very important for ensuring food security in my country and the world. Significance. Tuber shape is one of the important agronomic traits of potato, and it is also one of the important basis for identifying the characteristics of varieties. Different consumption habits and processed products have different requirements for potato shapes. For example, consumers in Northeast China prefer round potato-shaped varieties, while consumers in Central Plains and southern regions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 金黎平徐建飞朱文文李广存庞万福卞春松段绍光刘杰
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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