Method and system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii

A flavonoid carbon glycoside compound and the technology of Desmodium sativa, applied in the field of phytochemistry, to achieve the effects of simple process operation, high purity and high product yield

Active Publication Date: 2015-11-25
WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods and systems for extracting and separating Vicenin-2 need to be improved urgently

Method used

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  • Method and system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii
  • Method and system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii
  • Method and system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Figure 8 Show the general flow process of the inventive method, comprise following:

[0042] 1. Raw material processing: select 45,000-50,000 parts by weight of the leguminous plant Desmodium glabra, cut into 5-10 cm sections, wash off the sediment with drinking water, drain, and feed.

[0043] 2. Ethanol extraction: 80% ethanol reflux extraction twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, recover the ethanol until it has no alcohol smell.

[0044] 3. Concentration under reduced pressure: add water to dilute the extract concentrate to 5 times the volume of the medicinal material; filter through a 200-mesh filter cloth; get the column liquid; pack the net product resin that has been treated with 95% ethanol in advance, and replace it with purified water; The upper column liquid is pumped into the column for adsorption, and the flow rate is controlled to be 0.5-1 times BV / h. ...

Embodiment 2

[0067] 1. Carry out according to the steps 1-3 of Example 1 to obtain the Guangjin bulk drug.

[0068] 2. Multi-stage chromatographic separation and identification

[0069] (1) 300-400 parts by weight of Guangjin crude drug is separated by AB-8 macroporous resin chromatography, i.e. chromatographic separation I, ethanol-water mixed solvent 25:75, 35:65, 45:55, 65:35 gradient washing Take off, wash each gradient until there is no color, combine the fractions to get 25%, 35%, 45%, and 65% in four parts.

[0070](2) Use ODS reversed-phase medium-pressure chromatography to separate 35% of the chromatogram I, that is, chromatographic separation II. Specifically: the conditions are 8% isocratic for 30 minutes, 8-30% gradient for 200 minutes, and the peak is produced according to the chromatogram Sequentially, collect in fractions, collect one fraction per 100-150 volume fractions, collect a total of 40-50 fractions, combine the same fractions after HPLC detection, and obtain 3 merg...

Embodiment 3

[0078] 1. Carry out according to the steps 1-3 of Example 1 to obtain the Guangjin bulk drug.

[0079] 2. Multi-stage chromatographic separation and identification

[0080] (1) 300-400 parts by weight of Guangjin crude drug is separated by AB-8 macroporous resin chromatography, that is, chromatographic separation I, ethanol-water mixed solvent 20:80, 30:70, 40:60, 60:40 gradient washing Take off, wash each gradient until there is no color, combine the fractions to get 20%, 30%, 40%, and 60% in four parts.

[0081] (2) Separating 30% of the part obtained by chromatography I with ODS reverse-phase medium-pressure chromatography, that is, chromatographic separation II, specifically: use ethanol-water, the condition is 12% isocratic for 30 minutes, and the gradient of 12-30% is eluted for 200 minutes. According to the order of the peaks in the chromatogram, collect in portions, collect one portion per 100-150 volume parts, collect 40-50 fractions in total, combine the same fracti...

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Abstract

The invention provides a method and a system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii. The compound monomers are schaftoside, luteolin-6-C-beta-D-glucopyranose-8-C-beta-xylopyranoside and Vicenin-2. The method comprises the following steps: carrying out reflux extraction on herba desmodii styracifolii by using ethanol to obtain herba desmodii styracifolii extract; and carrying out multi-stage chromatographic separation on the extract by using an alcohol system, wherein the step comprises: carrying out first chromatographic separation on the extract by using a first alcohol system to obtain a first separated part; carrying out second chromatographic separation on the first separated part by using a second alcohol system; and sequentially obtaining three compound monomers according to the sequence of detection peaks. The invention provides a system for implementing the method. According to the method and/or system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii provided by the invention, the process is simple to operate, the production time is short, and the product is high in yield and high in purity.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and relates to a method and system for simultaneously extracting and separating multiple monomeric compounds in plants. Specifically, the invention relates to a method for extracting and separating three flavonoid carbon glycoside compound monomers from Desmodium sativa and system. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the genus Desmodium styracifolium (Osbeck) Merr. of the leguminous family. Its medicinal part is the dry aerial part, and its main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effects of clearing away heat and dehumidification, diuresis and treating stranguria. It is used for hot shower, sand shower, stone shower, astringent and painful urination, edema and oliguria, jaundice, red urine, and urinary calculi. [0003] Schaftoside, also known as 6-C-glucose-8-C-arabinose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/14
CPCC07D407/14
Inventor 王学海李莉娥许勇杨仲文冯芸尹海龙杨婷余通黄璐杨成兵黄天赐曹儒宾谢长
Owner WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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