A treatment solution for removing red blood cells and its application

Abstract

The invention discloses erythrocyte removing treating fluid and an application. One liter of the erythrocyte removing treating fluid comprises alcohol with the volume accounting for 15% to 30% of the whole volume, 5 g to 10 g of sodium chloride, 0.01 M to 0.1 M of phosphate buffers, 1 g to 5 g of glacial acetic acid, 1 g to 5 g of anticoagulants, 1 g to 6 g of surfactants and double distilled water. The erythrocyte removing treating fluid is low in cost and safe, injuries to cells can be reduced as far as possible, and the integrity of pathological cells can be guaranteed while erythrocytes are removed. When the cells are treated through the erythrocyte removing treating fluid, only the erythrocytes are split, only hemoglobin is removed, lesion cells and normal cells are not injured, the lesion cells are kept to the maximum degree, the shapes of the cells are maintained, the section making effect is good, the background is simple and clear, and results can be easily judged.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a treatment solution for removing red blood cells and its application. Background technique [0002] When clinical epithelial tissue is sampled, bloody samples will be formed if the sampling is inadvertent or the sampling site is damaged and causes bleeding. During ascites cytology examination, ascitic fluid will inevitably contain a large amount of blood during the process of extracting ascites, and bloody samples will also be formed. During the lavage sampling process of bronchi and other parts, there may also be bleeding, and bloody samples will also be formed. Bloody samples seriously affect the observation during the production process, mainly manifested in: a large number of red blood cells adhere to the production area, and even cover pathological cells, affecting pathological cells Interpretation of red blood cell fragments, agglomerated small clumps of red blood cel...

AI Technical Summary

Problems solved by technology

[0003] Among the three methods, the main feature of method 1 and method 2 is to achieve the effect of lysing red blood cells by adding a large amount of glacial acetic acid, and the lysis is complete, but a large amount of high-concentration glacial acetic acid will also lyse some normal cells and diseased cells. , so when red blood cells are removed, the diseased cells may also be lysed, resulting in missed detection

At the same time, cells at different sampling sites may be lysed due to overacid caused by high concentration of glacial acetic acid

Method 3 uses a hemolytic agent to lyse red blood cells. The hemolytic agent currently available on the market has a strong hemolytic effect, but contains highly toxic components such as potassium cyanide, which is a great threat to the operator and the environment.

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