Preparation method and application of tea fermentation bacterium yeast
A technology of tea fermentation and tea, which is applied in the field of microbiology and food processing, and the preparation of tea fermentation koji. It can solve the problems of unfavorable saccharomyces, lack of stability, and unsatisfactory products, so as to achieve high work intensity and high production efficiency. The long-term effect
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Embodiment 1
[0031] A preparation method for tea fermented koji, comprising the steps of:
[0032] (1) After pulverizing the tealeaves containing the fungus, use the dilution coating method to separate and purify the colony of the fungus, weigh 15g of tea and put it into a 250ml triangular flask, and adjust the moisture content of the tea to 80% with nutrient solution , after stirring evenly, use a high-pressure steam sterilizer to sterilize the tea leaves in the triangular flask at 0.1MPa and 121°C for 20 minutes, wait for the tea leaves in the triangular flask to cool to room temperature, and use an inoculation loop to pick out the loose cysts in a sterile environment Colonize the tea into the tea in the triangular flask and shake it well, then put it in the incubator for the first post-fermentation culture. The temperature in the incubator is kept at 26°C. After 4 days of cultivation, shake the flask once to make the newly grown mycelium distribute evenly , and then place the triangular...
Embodiment 2
[0044] A method for post-fermenting tea leaves by using the above-mentioned tea fermentation koji, comprising the following steps:
[0045] (1) Weighing raw tea leaves and adding water to adjust their water activity to 0.85 to obtain pretreated tea leaves;
[0046] (2) Determining the initial quantity of the phalloides in the pretreated tealeaves, the measurement result of this embodiment is that the initial quantity is lower than 10 3 / g, so the operation of step (3) is required,
[0047] (3) Take by weighing the tealeaves fermented koji whose weight is 0.5% of the pretreated tea leaves described in step (1), then add in the pretreated tea leaves and mix evenly to obtain the retreated tea leaves;
[0048] (4) Transfer the reprocessed tea leaves to the fermentation room for post-fermentation. During the post-fermentation process, the central temperature of the tea leaves is kept at 22-28°C. Within this temperature range, when the water activity is 0.85-0.87, the The growth r...
Embodiment 3
[0059] A preparation method for tea fermented koji, comprising the steps of:
[0060] (1) After pulverizing the tealeaves containing the spore-forming bacteria, separate and purify the spore-forming bacteria colonies on the DG18 medium by the dilution coating method, weigh 20 g of the tea leaves after heaping and put them into a 250 ml triangular flask, and use the nutrient solution Adjust the moisture content of the tea leaves to 90%, stir evenly, and then use a high-pressure steam sterilizer to sterilize the tea leaves in the triangular flask at 0.1MPa and 121°C for 20 minutes. Use an inoculation loop to pick up the colony of the fungus in the tea in the triangular flask and shake it well, then put it in the incubator for the first post-fermentation culture, keep the temperature in the incubator at 28°C, and shake the flask once after 5 days of cultivation Make the newly grown mycelium distribute evenly, and then place the triangular flask obliquely so that the tea leaves in...
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