Making method of monascus and tartary buckwheat functional food and application thereof
A functional food, the technology of red yeast rice tartary buckwheat, applied in the field of bioengineering, can solve the problems of ignoring the overall efficacy of red yeast rice, not investigating, and not involving hypoglycemic efficacy, etc.
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Embodiment 1
[0045] Embodiment 1 A kind of red yeast rice tartary buckwheat functional food preparation method
[0046] The appearance of the red yeast tartary buckwheat functional food is pink to purple, with a unique fermented aroma, containing 2-10 mg / g of flavonoids, 0.5-5 mg / g of lovastatin, and 0.4-6 mg / g of monascus pigment; The above-mentioned red koji tartary buckwheat functional food is prepared by using tartary buckwheat as the main raw material and Monascus as the strain, through test tube expansion culture, liquid shake flask culture, seed tank seed culture and solid fermentation culture.
Embodiment 2
[0048] A preparation method of red yeast tartary buckwheat functional food, said method comprising the steps of:
[0049] a. Test tube expansion culture: inoculate Monascus strains in potato glucose medium, cultivate at a temperature of 20°C for 10 days, and store at 1°C to obtain Monascus test tube slant spores;
[0050]b. Liquid shake flask culture: Inoculate a ring of Monascus test tube slant spores into a 250mL Erlenmeyer flask filled with 20mL liquid shake flask culture medium. Cultivate for 18 hours to make liquid shake flask strains; wherein the weight of each component of the raw materials contained in each 1L medium is: bran 5g, glucose 5g, peptone 1g, yeast extract 2g, magnesium sulfate 0.01g, dipotassium hydrogen phosphate 0.01g, the medium was sterilized at 100°C for 60 minutes.
[0051] c. First-level seed tank seed culture: Inoculate liquid shake flask strains into seed tank culture medium at an inoculum size of 1%, at a temperature of 20°C, a stirring speed of ...
Embodiment 3
[0054] A preparation method of red yeast tartary buckwheat functional food, said method comprising the steps of:
[0055] a. Test tube expansion culture: inoculate Monascus strains in potato glucose medium, cultivate them at a temperature of 20°C for 5 days, and store them at 4°C to obtain Monascus test tube slant spores;
[0056] b. Liquid shake flask culture: Inoculate a ring of Monascus test tube slant spores into a 250mL Erlenmeyer flask filled with 20mL liquid shake flask culture medium. Cultivate for 40 hours to make liquid shake flask strains; the weight of each component of the raw materials contained in each 1L medium is bran 5g, glucose 8g, peptone 2g, yeast extract 3.5g, magnesium sulfate 0.05g, dipotassium hydrogen phosphate 0.05g, the culture medium was sterilized at 110°C for 50 minutes;
[0057] c. First-level seed tank seed culture: Inoculate liquid shake flask strains into the seed tank culture medium at an inoculum size of 1%, at a temperature of 28°C, a sti...
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