Flavones separated and purified from shepherd's purse, and preparation method and application thereof

A flavonoid compound, separation and purification technology, applied in the field of 5-hydroxymethyl neoflavonoid compound and preparation thereof, and achieves the effects of low preparation cost, novel structure and good development and utilization value

Inactive Publication Date: 2015-12-02
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no literature and patent reports about the flavonoids extracted from shepherd's purse and their activities at home and abroad.

Method used

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  • Flavones separated and purified from shepherd's purse, and preparation method and application thereof
  • Flavones separated and purified from shepherd's purse, and preparation method and application thereof
  • Flavones separated and purified from shepherd's purse, and preparation method and application thereof

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preparation example Construction

[0026] The preparation method of this flavonoid compound comprises the following steps:

[0027] (1) Take dried aerial part of shepherd's purse as raw material, after crushing, add ethanol solution with a mass concentration of 70% to it for ultrasonic extraction for 20-40min, filter after extraction, collect filtrate, and add Ethanol solution with a mass concentration of 70%, repeat the above-mentioned ultrasonic extraction and filtration operations twice, combine all filtrates, concentrate under reduced pressure until there is no solvent, and extract the extract for future use;

[0028] (2) Add distilled water to the extraction extract obtained in step (1), and obtain a dispersion liquid after ultrasonic dispersion, and then sequentially extract the dispersion liquid with petroleum ether, ethyl acetate and n-butanol, and extract each part of the obtained The liquid was concentrated under reduced pressure to obtain the petroleum ether part, the ethyl acetate part, the n-butano...

Embodiment 1

[0033] Get 11.0kg dried shepherd's purse aerial part as raw material, after pulverizer pulverizes, add mass concentration in raw material powder and be 70% ethanol solution and carry out ultrasonic extraction 3 times, half an hour each time, merge all filtrates after extraction finishes, reduce through low temperature Concentrate under pressure until there is no solvent to obtain 1100 g of extract extract, which is ultrasonically dispersed and suspended in distilled water. After that, the dispersion is extracted with petroleum ether, ethyl acetate and n-butanol successively, and each part of the obtained The extract was concentrated under reduced pressure to obtain 108.5g of petroleum ether, 237.0g of ethyl acetate, and 320.7g of n-butanol. Combining with the previous pharmacological screening results, the active part of shepherd's purse-ethyl acetate was separated in a targeted manner. Ultrasonically disperse the obtained ethyl acetate parts in water, and after filtering, pass...

Embodiment 2

[0035]Take 11.0kg of dried shepherd's purse aerial part as raw material, pulverize after pulverizer, add the ethanol solution that mass concentration is 70% to raw material powder and carry out ultrasonic extraction 3 times, each time 20min, after extraction finishes, combine all filtrates, through low temperature depressurization Concentrate until there is no solvent to obtain 1100 g of extract extract, which is ultrasonically dispersed and suspended in distilled water. After that, the dispersion is extracted with petroleum ether, ethyl acetate and n-butanol in sequence, and each part of the obtained extract is extracted The liquid was concentrated under reduced pressure to obtain 106.8g of petroleum ether, 246.2g of ethyl acetate, and 325.1g of n-butanol. Combining with the previous pharmacological screening results, the active part of shepherd's purse-ethyl acetate was separated, and the The obtained ethyl acetate part is ultrasonically dispersed in water, and after filtrati...

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Abstract

The invention relates to flavones separated and purified from shepherd's purse. The method comprises the following steps: ethanol ultrasonic extraction, solvent extraction, silica gel column chromatography eluting separation, resin column adsorption, silica gel column chromatography eluting separation, vacuum concentration, vacuum drying and the like. The name of the separated flavones is 2-(3,4-dimethoxyphenyl)-5-(hydroxymethyl-7-O-beta-D-glucosyl-4H-chromene-4-one. The raw material, which is the medicinal and edible shepherd's purse, has abundant sources and is cheap and accessible. The prepared 5-hydroxymethyl new flavones have novel structure, have obvious effect of inhibiting tumor cell proliferation, and can be used as a cell proliferation inhibitor or antitumor agent for antitumor research. The flavones have favorable development and utilization values, and lay foundation for research and development of clinical drugs for preventing and treating tumors in future.

Description

technical field [0001] The invention relates to the technical field of extraction and preparation of flavonoids, in particular to a 5-hydroxymethyl neoflavone compound obtained by extraction and separation from Cruciferae shepherd's purse and its preparation method and application. Background technique [0002] Flavonoids are a class of important biologically active substances that widely exist in the plant kingdom, and have anti-oxidation, anti-aging, anti-virus, anti-tumor, and antibacterial effects. The antioxidant capacity of organisms is closely related to its disease resistance, stress resistance and anti-aging, so finding effective antioxidants from natural plants for use in medicine, food, health products, cosmetics, etc. is one of the current research hotspots. [0003] shepherd's purse ( Capsella bursa-pastoris ), also known as Hushengcao, Qingmingcao, Blood Pressure Grass, Zongzicai, etc. It is an annual or biennial herbaceous plant of the genus Shepherd's Purse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61P35/00
Inventor 马勤阁魏荣锐桑志培何朝政罗保民柳文敏周亮段翔耀李亚萍黄红春
Owner NANYANG NORMAL UNIV
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