Pseudomonas phosphate dissolving bacterium Y2, bioorganic fertilizer prepared through using bacterium, and application of fertilizer

A technology of Pseudomonas and bio-organic fertilizer, which is applied in the field of agricultural microorganisms, can solve problems such as difficulty in exerting activity and competition of phosphate-solubilizing bacteria, and achieve good growth-promoting effect, promote crop growth, and reduce application

Active Publication Date: 2015-12-02
NANJING AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus solubilizing bacteria in the rhizosphere of plants are not enough to

Method used

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  • Pseudomonas phosphate dissolving bacterium Y2, bioorganic fertilizer prepared through using bacterium, and application of fertilizer
  • Pseudomonas phosphate dissolving bacterium Y2, bioorganic fertilizer prepared through using bacterium, and application of fertilizer
  • Pseudomonas phosphate dissolving bacterium Y2, bioorganic fertilizer prepared through using bacterium, and application of fertilizer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, isolation and identification of functional strains

[0037] Cut 5g of potato roots into a triangular flask filled with 45mL of sterile water, vibrate at 170rpm for 30min to make a suspension, and then prepare a concentration of 10 -3 、10 -4 、10 -5 、10 -6 soil dilution. from 10 -4 、10 -5 、10 -6 Take 0.1mL of the diluted solution and spread it on calcium phosphate salt inorganic phosphorus medium and egg yolk medium respectively (each concentration is repeated 3 times on each medium). Separation and decomposition of organic phosphorus and inorganic phosphorus bacteria plates were cultured at 30°C for 3d and 5d, respectively, and were taken out for observation. The colony that produced a transparent circle on the solid medium plate was initially identified as phosphate-solubilizing bacteria. Refrigerator storage is reserved for further study. Finally, a strain was isolated and named Y2, which had excellent phosphorus-dissolving effect.

[0038] Where...

Embodiment 2

[0042] The production of embodiment 2, Y2 fermented liquid

[0043] Inoculate strain Y2 (CGMCC NO.10242) into LB culture medium for liquid fermentation production, the conditions for fermentation production are: pH 6.0, fermentation temperature range 30-35°C, stirring speed 200 rpm, fermentation time 48h, Make the bacteria content in the fermentation broth ≥ 1×10 10 a / ml;

[0044] The preparation method of the LB medium used is as follows, taking the preparation of 1L medium as an example: 10g of peptone, 5g of yeast powder, 10g of NaCl, 18-20g of agar, constant volume to 1000mL, natural pH value, sterilized at 121°C for 20min.

Embodiment 3

[0045] Embodiment 3, greenhouse growth-promoting effect test

[0046] 3.1 The application effect of Y2 strain on potatoes (first season, second season)

[0047] Both potato growth-promoting experiments were carried out in the greenhouse of Jiangsu Zhongyi Biological Fertilizer Engineering Center. The tested crop: the potato variety was Kexin No. 1. Potato seedlings are provided by local farmers. There are three treatments: 1) adding decomposed chicken manure, calcium phosphate and Y2 fermentation broth (Y2); 2) adding decomposed chicken manure and calcium phosphate (CK1); 3) adding decomposed chicken manure and superphosphate (CK2). Each treatment has 8 pots, each pot is filled with 2kg of soil, the amount of fertilizer added is 30g per pot, the amount of calcium phosphate and superphosphate added is 3g / pot, and the fertilizer and soil are mixed well as the base fertilizer. The amount of inoculation was 3% (ml / 100g, the same below). In this embodiment, the following experim...

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Abstract

The invention discloses a Pseudomonas phosphate dissolving bacterium Y2, a bioorganic fertilizer prepared through using the bacterium, and an application of the fertilizer. The above bacterium strain is separated from potato rhizosphere, and is preserved in China General Microbiological Culture Collection Center on Dec. 25, 2014 with the preservation number of CGMCC NO.10242. A result of rocking bottle experiments show that the bacterium strain can effectively reduce the pH value of a solution and dissolve insoluble phosphate in the solution; and a result of bacterium filled pot test shows that the bacterium strain can effectively promote growth of potato, corn and tobacco plants, and can massively colonize at the rhizosphere of the plants to realize a growth promotion effect. The granular bioorganic fertilizer developed through using the bacterium strain, decomposed chicken manure and rapeseed meal can be applied to substantially promote the growth of the corn plants.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms and provides a high-efficiency phosphorus-solubilizing bacterium Y2 belonging to the genus Pseudomonas and a bio-organic fertilizer developed therefrom. Background technique [0002] Phosphorus is an essential nutrient element for plant growth. Phosphorus deficiency is common in cultivated land in my country. Ineffective phosphorus forms account for 95% of the soil, and plants can hardly absorb and utilize it directly. Insoluble phosphorus in soil can be divided into organic phosphorus and inorganic phosphorus, among which insoluble organic phosphorus accounts for 20%-50% of total soil phosphorus. The available phosphorus content of phosphorus fertilizers such as superphosphate is low, and the seasonal utilization rate of phosphorus fertilizers after application is 5%-25%, and most of the remaining phosphorus is mixed with Ca in the soil. 2+ , Fe 3+ and Al 3+ etc. combined to form ins...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C05F17/00A01N63/00A01P21/00C12R1/38
CPCY02W30/40
Inventor 沈其荣李荣赵买琼张瑞福刘小玉沈标
Owner NANJING AGRICULTURAL UNIVERSITY
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