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Application of schisandrin in preparing medicine for improving glucocorticoid-induced osteoporosis

A technology of schisandra C and glucocorticoid, applied in the field of medicine, can solve the problem of lack of research, and achieve the effects of improving osteoporosis, inhibiting osteoblast apoptosis, and promoting osteoblast proliferation

Inactive Publication Date: 2015-12-09
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Schisandrin C (schisandrin C) (see formula 1 for structural formula) exists in a small amount in Schisandra medicinal materials, and there are few studies at present
Schisandra C on the regulation of osteoblasts, and its research on the prevention / treatment of glucocorticoid-induced osteoporosis has not been reported in the literature

Method used

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  • Application of schisandrin in preparing medicine for improving glucocorticoid-induced osteoporosis
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  • Application of schisandrin in preparing medicine for improving glucocorticoid-induced osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 (Schisandra C improves the study of dexamethasone-induced osteoporosis in mice)

[0030] (1) Materials and methods

[0031] (1) Experimental animals

[0032] 40 adult male ICR mice (8 weeks old, with an average body weight of 30±2g) were provided by the Experimental Animal Center of Second Military Medical University. Raised in the SPF animal experiment center, 12 hours a day, day and night, constant temperature 18 ~ 20 ℃, mice can eat and drink freely. All mice were reared for one week in advance, and the experiment was carried out after adapting to the living environment.

[0033] (2) Preparation of main reagents

[0034] 1. Dexamethasone sodium phosphate: use 0.9% sodium chloride solution to prepare a solution with a concentration of 0.25 mg / ml;

[0035] 2. Schisandra C: Dissolve 20mg of Schisandra C in 2100ml of absolute ethanol, pipette until completely dissolved; dissolve 20mg of completely dissolved Schisandra C in 84ml of propylene glycol, the fina...

Embodiment 2

[0048] Example 2 (the effect of Schisandra C combined with dexamethasone on the proliferation and differentiation of mouse osteoblasts)

[0049] (1) Materials and methods

[0050] Cell line: mouse osteoblast cell line (MC3T3-E1) was purchased from ATCC. The above cell lines were incubated in α-MEM medium containing 10% fetal bovine serum at 37°C with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, replace the medium for 48 hours, when the cell growth reaches saturation, digest with 0.25% trypsin + 0.02% EDTA for passage, once every 2-3 days, the experiment uses logarithmic growth phase cells.

[0051] 1. The effect of Schisandra C on the proliferation inhibition of mouse osteoblasts induced by dexamethasone

[0052] 1.1. The osteoblasts MC3T3-E1 in the logarithmic growth phase were inoculated in three 96-well plates, and the cell density was adjusted to about 5×10 3 Each culture plate is divided into 7 groups, namely ① blank control...

Embodiment 3

[0071] Embodiment 3 (preparation of schisandra acetin tablet)

[0072] Take 25g of schisandra acetone, 10g of hydroxypropyl methylcellulose, 50g of microcrystalline cellulose, 5g of croscarmellose sodium, mix well, add an appropriate amount of 60% ethanol to make a soft material, pass through a 24-mesh sieve and granulate , dried at 50°C for 2 hours, the dried granules were passed through a 30-mesh sieve for granulation, 2.5g of magnesium stearate was added, mixed evenly, and pressed into 1000 tablets respectively.

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Abstract

The invention relates to new medical application of schisandrin, i.e., the application in preparing medicine for preventing or treating glucocorticoid-induced osteoporosis. The application has the advantages that shown by in-vitro and in-vivo research for the first time, the schisandrin has the effects of obviously improving osteoporosis caused by long-time use of dexamethasone and enhancing osteoblast proliferation, and has the prospect for application in preparing the medicine for preventing or treating glucocorticoid-induced osteoporosis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the new medical application of schisandra C, that is, the application of schisandra C in the preparation of drugs for the prevention / treatment of glucocorticoid-induced osteoporosis. Background technique [0002] Glucocorticoids (glucocorticoid, GC) are a class of drugs widely used clinically for the treatment of many chronic inflammatory diseases and autoimmune diseases, such as ulcerative colitis, systemic lupus erythematosus, asthma, rheumatoid arthritis etc. However, long-term application of GC often causes many side effects, among which glucocorticoid-induced osteoporosis (GIO) is one of the most serious side effects caused by GC. GIO is the most common secondary osteoporosis and can cause osteoporotic fractures. It is estimated that about 30% of patients who receive GC treatment for more than 6 months will develop osteoporosis; while about 30% to 50% of adult patients wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/36A61P19/10
Inventor 程彬彬凌昌全程思路杜娟梁淑芳邓正亭姚曼郭玉玉孟永斌
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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