Muscovy duck parvovirus VP3 genetic recombination fowl pox virus transfer vector and building method thereof

Abstract

The invention discloses a Muscovy duck parvovirus VP3 genetic recombination fowl pox virus transfer vector and a building method thereof. The method includes: fowl pox virus genome is used as the template to amplify the homologous recombination arms TKL and TKR of TK genes, the TKL and TKR are connected into a TK fragment with multiple cloning sites (MCS), and the TK fragment is connected into a framework plasmid to obtain the plasmid pTK; a reverse serial connection expression box S is synthesized and inserted into the plasmid pTK to obtain the plasmid pTKS; VP3 genes are inserted into an MSC1 position behind an early promotor P7.5, and EGFP BGH pA is inserted into an MCS2 position behind a late promotor P11 to obtain the target transfer vector pTKS-VP3-EGFP. After the pTKS-VP3-EGFP transfects chicken embryonic fibroblast infected by the fowl pox virus, RT-PCR and fluorescent protein detection show that VP3 can be expressed normally. By the Muscovy duck parvovirus VP3 genetic recombination fowl pox virus transfer vector and the building method thereof, a foundation is laid on the further development of safe and efficient MDPV recombinant fowl pox virus vaccines.

Description

Technical field [0001] The present invention is a biotechnology field, involving a duck small virus VP3 gene recombinant chicken acne virus transfer carrier and its construction method. Background technique [0002] Hya duck fine virus is a kind of acute and sewage infectious disease caused by Muscovyduckparvovirus (MDPV). It is characterized by high contagion and mortality. It is the most serious harm in the duck breeding industry at present.One of the infectious diseases.MDPV mainly invasion of ducks within 3 weeks, also known as "three weeks of disease".The incidence of Hya Duck is 20 % to 60 %, and the mortality rate of the disease ranges from 20 % to 40 %; the incidence and mortality of ducks above 30 days are low, and the sick ducks often become stiff ducks.The occurrence of this disease has no seasonality, and the disease can be developed all year round, focusing on prevention.At present, there is a weak toxic vaccine that prevents the disease. The immune originality and g...

AI Technical Summary

Problems solved by technology

The attenuated vaccine is easily interfered by the maternal antibody level, which affects the immune prevention effect of young muscovy ducks, and the attenuated vaccine also has the risk of returning to strong virulence

Images