Method for determination of content of kappa-casein in cow milk

A technology of casein and milk, applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve problems that need to be improved, and achieve the effect of high repeatability, strong accuracy, and short detection time

Active Publication Date: 2015-12-09
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, current methods for the determina

Method used

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  • Method for determination of content of kappa-casein in cow milk
  • Method for determination of content of kappa-casein in cow milk
  • Method for determination of content of kappa-casein in cow milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] In this example, the kappa-casein content in raw milk was determined by the following steps:

[0078] (1) Raw milk pretreatment

[0079] Take 30ml of raw milk to be tested, put it in a 50ml centrifuge tube, and centrifuge at 6000r / min for 20 minutes to remove milk fat; then put it in a -40°C refrigerator for 20 minutes; take it out and return it to room temperature, and use 40mmol / L acetic acid aqueous solution to adjust the pH to 4.6, centrifuge at 6000r / min for 20 minutes, discard the supernatant, wash with water 3 times, centrifuge at 6000r / min for 20 minutes, discard the supernatant, and then wash with 40mmol / L boric acid The pH of the solution was adjusted to 6.6 to prepare the stock solution to be tested. Take 10ml of the stock solution to be tested, add 3.6g of urea and 0.05g of dithiothreitol (DTT), shake and mix to prepare the solution to be tested.

[0080] (2) Activation of uncoated capillary

[0081] Cut off an uncoated quartz capillary column with an in...

Embodiment 2

[0089] In this example, the kappa-casein content in raw milk was determined by the following steps:

[0090] (1) Raw milk pretreatment

[0091]Take 30ml of raw milk to be tested, put it in a 50ml centrifuge tube, and centrifuge at 3000r / min for 30 minutes to remove milk fat; then place it in a -40°C refrigerator for 20 minutes; take it out and return it to room temperature, and use 40mmol / L acetic acid aqueous solution to adjust the pH to 4.6, centrifuge at 3000r / min for 30 minutes, discard the supernatant, wash with water 3 times, centrifuge at 3000r / min for 30 minutes, discard the supernatant, and then wash with 40mmol / L boric acid The pH of the solution was adjusted to 6.6 to prepare the stock solution to be tested. Take 10ml of the stock solution to be tested, add 3.6g of urea and 0.05g of dithiothreitol (DTT), shake and mix to prepare the solution to be tested.

[0092] (2) Activation of uncoated capillary

[0093] Cut off an uncoated quartz capillary column with an i...

Embodiment 3

[0101] In this example, the kappa-casein content in raw milk was determined by the following steps:

[0102] (1) Raw milk pretreatment

[0103] Take 30ml of raw milk to be tested, put it in a 50ml centrifuge tube, and centrifuge at 10000r / min for 10 minutes to remove milk fat; then put it in a -40°C refrigerator for 10 minutes; take it out and return it to room temperature, and use 40mmol / L acetic acid aqueous solution to adjust the pH to 4.6, centrifuge at 3000r / min for 30 minutes, discard the supernatant, wash with water three times, centrifuge at 10000r / min for 10 minutes, discard the supernatant, and then wash with 40mmol / L boric acid The pH of the solution was adjusted to 6.6 to prepare the stock solution to be tested. Take 10ml of the stock solution to be tested, add 3.6g of urea and 0.05g of dithiothreitol (DTT), shake and mix to prepare the solution to be tested.

[0104] (2) Activation of uncoated capillary

[0105] Cut off an uncoated quartz capillary column with...

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Abstract

The invention provides a reagent for coating layer processing on a capillary and a method for determination of the content of kappa-casein in cow milk. According to the embodiment of the invention, the capillary is used for determination of the content of kappa-casein in the cow milk through a capillary electrophoresis method; the reagent contains the following aqueous solutions: 200 mmol/L tris(hydroxymethyl)aminomethane; 120 mmol/L hexadecyl ammonium bromide; 80 mmol/L polyethylene glycol, and 60 mmol/L pentanediamine, wherein the pH value of the reagent is 8.5. Therefore, the method for determination of the content of kappa-casein by using the capillary obtained by using the reagent for coating layer processing on the capillary has at least one following advantages of good separation effect, short detection time, high repeatability and strong accuracy.

Description

technical field [0001] The invention relates to the field of food. In particular, the present invention relates to a method for determining the content of kappa-casein in bovine milk. More specifically, the present invention relates to a reagent for coating capillaries and a method for determining the content of kappa-casein in milk. Background technique [0002] κ-casein is the least protein in the casein components, accounting for about 12% of the total casein and 10% of the total protein in milk. The relative molecular mass of κ-casein is about 19000D, containing 169 amino acid residues and 1 phosphate group. [0003] However, the current method for determining the content of κ-casein in milk still needs to be improved. Contents of the invention [0004] The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, an object of the present invention is to provide a reagent for coating capillaries and a method for de...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 刘宇陈伟史玉东陈云母智深
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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