Method for separation and identification of kenaf root knot nematode

A root-knot nematode and identification method technology, applied in the field of microorganisms, can solve problems such as harming kenaf, affecting the yield and quality of kenaf, and achieving the effects of strong accuracy, simple operation and high purity

Inactive Publication Date: 2015-12-16
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

Through investigation, it is found that root-knot nematodes mainly harm the roots of kenaf, forming root knots and nodules, which dire

Method used

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  • Method for separation and identification of kenaf root knot nematode
  • Method for separation and identification of kenaf root knot nematode
  • Method for separation and identification of kenaf root knot nematode

Examples

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Embodiment 1

[0017] (1) Morphological identification: select typical root-knot nematode diseased plants of kenaf, and use the five-point sampling method to collect about 500g of the host root system and the surrounding 5-20cm deep plow layer soil. Under greenhouse conditions, the diseased roots and diseased soil were inoculated on potted tomatoes cultivated in sterilized soil for 45 days in advance for purification and cultivation; under a stereomicroscope, female worms and egg masses were picked out from the inoculated tomato roots with a dissecting needle, and the Eggs were placed in a petri dish filled with distilled water and hatched for 4-5 days to obtain J2. The male worms in the soil were separated by the Berman funnel method; like figure 1 Shown: A, B, C, D are the perineal patterns of M. incognita, M. elegans, M. javanica and M. peanut respectively;

[0018] (2) Analysis and identification of isoenzymes: Using conventional polyacrylamide gel electrophoresis technology, the purifi...

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Abstract

The invention provides a method for separation and identification of kenaf root knot nematode and belongs to the technical field of microorganisms. The technical scheme is that (1) classical morphological identification; (2) isozyme analysis and identification; (3) molecular biological identification: the PCR identification is performed through primers 5-GGTCAATGTTCAGAAATTTGTGG-3 and 5-TACCTTTGACCAATCACGCT-3, COll genes and lrRNA genes thereof located in mitochondrion DNAs are amplified, and sequences are subjected to comparative analysis and identification. The physiological race of the root knot nematode is made clear. The method integrates separation, purification and identification, a method of combining morphology, isozyme and molecular biology is adopted for identification and verification, the result shows that the molecular biological identification is high in efficiency and accuracy and suitable for field sampling fast separation, and the biological characteristics of insect body wild type population or the physiological race are identified.

Description

technical field [0001] The invention relates to a method for isolating and identifying root-knot nematodes of kenaf, belonging to the technical field of microorganisms. Background technique [0002] Kenaf is an annual fiber crop belonging to Malvaceae Hibiscus and is one of the second largest natural fiber crops in the world after cotton. It is widely cultivated in India, Bangladesh, China, Thailand and other countries. Kenaf fiber has excellent characteristics such as high yield, strong hygroscopicity, good heat dissipation, and easy degradation, so it has attracted the attention of countries all over the world. In recent years, it has attracted the attention of enterprises as an excellent raw material for papermaking, textile and biomass energy, hemp plastic and hemp carbon. [0003] With the continuous deepening of research, kenaf is widely used in various fields such as textiles, construction, home furnishing, and automobile industry. In order to meet people's growing ...

Claims

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Application Information

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IPC IPC(8): A01K67/033C12Q1/44C12Q1/32C12Q1/68
CPCA01K67/0336C12Q1/32C12Q1/44C12Q1/6888G01N2333/904G01N2333/916
Inventor 牛小平祁建明陈绵才王会芳徐建堂陶爱芬林荔辉
Owner FUJIAN AGRI & FORESTRY UNIV
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