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Composition and application thereof, and preparation containing composition

A technology of a composition and a pharmaceutical preparation, applied in the field of medicine, can solve the problems such as the lack of A.

Inactive Publication Date: 2015-12-16
HANGZHOU HUAJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few developments and applications of G. purple-backed in pharmacy. At present, there are no related patents, and there are no related reports on the combined use of thymosin and G. purple-backed polysaccharides.

Method used

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  • Composition and application thereof, and preparation containing composition
  • Composition and application thereof, and preparation containing composition
  • Composition and application thereof, and preparation containing composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The thymosin extract is prepared by enzyme-assisted extraction, and the specific operation method is as follows:

[0067] (1) 1043 grams of freshly frozen calf thymus was removed from the fat and fascia, rinsed, weighed 1058 grams, added 1050 ml of deionized water, and homogenized for 20 minutes at room temperature until it became a milky white liquid.

[0068] (2) Add 1 mol / L hydrochloric acid to the above-mentioned milky white liquid, adjust the pH to 1.8, then add 0.1% pepsin according to the weight of the raw material, stir at 200 rpm, enzymatically extract at 40°C for 3 hours, and then denature at 100°C for 15 Minutes, cooled to room temperature to obtain enzymatic hydrolysis solution.

[0069] (3) The enzymolysis solution was filtered with a 300-mesh non-woven fabric, and the filtrate was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 10,000 Daltons to obtain a slightly yellow, clear and transparent solution, which was the thymos...

Embodiment 2

[0078] The thymosin extract was prepared by an enzyme-assisted extraction method, and the specific operation method was as follows:

[0079] (1) 580 grams of freshly frozen calf thymus was removed from the fat and fascia, rinsed, weighed 576 grams, added 1150 ml of deionized water, and homogenized for 20 minutes at room temperature until it became a milky white liquid.

[0080] (2) Adjust the pH to 1.8 with 1 mol / L hydrochloric acid, add 0.2% pepsin according to the weight of raw materials, stir at 200 rpm, enzymatically extract at 40°C for 3 hours, then denature at 100°C for 15 minutes, and cool to room temperature to obtain Enzyme solution.

[0081] (3) The enzymolysis solution was filtered with a 300-mesh non-woven fabric, and the filtrate was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 10,000 Daltons to obtain a slightly yellow, clear and transparent solution, which was the thymosin extract.

[0082] Thymosin extract was purified by q...

Embodiment 3

[0090] The thymosin extract was prepared by enzyme-assisted extraction, and the specific operation method was as follows:

[0091] (1) 2000 grams of freshly frozen calf thymus was removed from the fat and fascia, rinsed, weighed 2038 grams, added 2000 ml of deionized water, and homogenized for 20 minutes at room temperature until it became a milky white liquid.

[0092] (2) Use 0.1 hydrochloric acid solution to adjust the pH of the milky white liquid to 1.8, add 0.1% pepsin according to the weight of raw materials, stir at 200 rpm, enzymatically extract at 50°C for 3 hours, then denature at 100°C for 15 minutes, and cool to room temperature. Obtain enzymatic solution.

[0093] (3) The above enzymolysis solution was filtered through a 300-mesh non-woven fabric, and the filtrate was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 10,000 Daltons to obtain a slightly yellow, clear and transparent solution, which was the thymosin extract.

[0094]...

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Abstract

The invention relates to the field of medicines, and in particular relates to a composition and an application thereof, and a preparation containing the composition. The composition comprises thymosin and begonia fimbristipula. Begonia fimbristipula polysaccharide is extracted and purified. An in vitro cell experiment and an animal experiment prove that the begonia fimbristipula polysaccharide has relatively high activity of stimulating immunization and inhibiting tumor cell growth. Furthermore, an animal experiment result proves that enhanced immunocompetence of the begonia fimbristipula polysaccharide and a thymosin extract has synergistic effects.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a composition and its application, and a preparation containing the composition. Background technique [0002] Polypeptides and polysaccharides are common active substances that can enhance immunity. Active polypeptides and polysaccharides have been developed into a variety of pharmaceutical preparations, which are widely used in the adjuvant treatment of autoimmune diseases, infectious diseases and inflammation-mediated diseases. good therapeutic effect. [0003] Thymosin is a commonly used immunotherapy drug in clinical practice. It is widely used in the treatment of viral hepatitis, as well as in the auxiliary treatment of autoimmune diseases (Vitiligo, rheumatoid arthritis, etc.) and infectious diseases such as sepsis, tuberculosis, and bronchitis. treat. [0004] Clinically used thymosin is mainly extracted from animal thymus tissue, and then made into different dosage forms. Curr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/22A61P37/04C08B37/00A23L1/09C12P21/06C07K1/22A61K36/28A61K31/715
Inventor 周正兵张治国周天琼傅诗丽
Owner HANGZHOU HUAJIN PHARMA
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