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Kit and detection method thereof

A kit and the technology of schizoglycin A, which are applied in the field of medical devices, can solve the problems of kit storage and carrying barriers, affect promotion and application, and cannot be frozen repeatedly, achieving excellent results, long storage period, and easy portability Effect

Inactive Publication Date: 2015-12-16
WUXI CHANGAN SHUGUANG GLOVE FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the active ingredient is a protein antibody, it cannot be frozen repeatedly, which brings great obstacles to the preservation and carrying of the kit, seriously affecting its promotion and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The test kit of this embodiment contains:

[0056] Primary anti-digoxigenin antibody (concentration is 5 μg / ml),

[0057] Secondary antibody mouse anti-goat IgG (concentration: 8×10 -7 mol / L),

[0058] 3H-labeled sarcoside A (concentration is 17Ci / mmol),

[0059] The mixed solution (mass ratio of the two is 1:0.25, and the mass fraction of trishydroxymethylaminomethane is 13%) and

[0060] Aqueous solution of polyethylene glycol 6000 (mass fraction 30%).

Embodiment 2

[0062] The test kit of this embodiment contains:

[0063] Primary anti-digoxigenin antibody (concentration is 15 μg / ml),

[0064] Secondary antibody mouse anti-goat IgG (concentration: 4×10 -7 mol / L),

[0065] 3H-labeled argoside A (concentration is 12Ci / mmol),

[0066] A mixed solution of Tris and disodium edetate (the mass ratio of the two is 1:0.5, and the mass fraction of Tris is 21%),

[0067] Aqueous solution of polyethylene glycol 6000 (mass fraction 23%) and argoside A (concentration gradient of 2nM, 5nM, 10nM, 20nM, 50nM, 0.1M and 0.2M).

Embodiment 3

[0069] The test kit of this embodiment contains:

[0070] Primary anti-digoxigenin antibody (concentration is 10 μg / ml),

[0071] Secondary antibody mouse anti-goat IgG (concentration: 6×10 -7 mol / L),

[0072] 3H-labeled argoside A (concentration is 7Ci / mmol),

[0073] The mixed solution (mass ratio of the two is 1:0.75, and the mass fraction of trishydroxymethylaminomethane is 28%) and

[0074] Aqueous solution of polyethylene glycol 6000 (mass fraction 15%).

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PUM

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Abstract

The invention relates to the technical field of medical equipment and discloses a kit and a detection method thereof. The kit comprises the following components: a primary-antibody digoxin antibody, secondary-antibody goat-anti-mouse IgG, 3H mark divaricoside, polyethylene glycol 6000, a 3H scintillation solution, a mixed solution of trishydroxymethyl aminomethane and ethylene diamine tetraacetic acid; and the kit contains divaricoside or not. The kit has extremely good resuscitation effect after being frozen and can be directly used after being unfrozen and resuscitated; the activity is not influenced when the kit is repeatedly frozen and the kit is very convenient to store and carry; and furthermore, the kit is simple to operate, has high flexibility and strong specificity, can be used for stably and reliably diagnosing eclampsia in a pregnancy period, and provides beneficial supports to subsequent therapy.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a kit for detecting preeclampsia or eclampsia during pregnancy and a detection method thereof. Background technique [0002] Preeclampsia during pregnancy is a syndrome with complex etiology. If left untreated, it can develop into eclampsia, which is life-threatening. The main symptoms are high blood pressure, proteinuria, edema, convulsions, renal function damage, etc. It has become a disease with the highest mortality rate among pregnant women in the world, and the specific etiology is currently unknown. Studies have shown that a substance produced by the placenta that causes maternal vascular endothelial dysfunction in susceptible women may be one of the reasons for the onset of preeclampsia. Elevated blood pressure is the most obvious hallmark of the disease, causing damage to the maternal endothelium, kidneys and liver, and releasing vasoconstrictors. [0003] Pree...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/53G01N2800/368
Inventor 缪建良
Owner WUXI CHANGAN SHUGUANG GLOVE FACTORY