A method for purifying pinolanic acid from pine nut oil
A technology for pinocyst acid and pine nut oil, applied in the field of biological extraction, can solve the problems of difficult recovery and reuse, small scope of application, expensive lipase, etc., and achieve the effects of easy preparation and low cost
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Embodiment 1
[0020] Include the following steps:
[0021] A. the saponification treatment of pine nut oil;
[0022] Take the pine nut oil of Korean pine, place it in 4 times the volume of 60% ethanol solution, add sodium hydroxide or potassium hydroxide with a mass fraction of 10% of the ethanol solution, stir at room temperature for 24 hours or heat to reflux for 0.5 hours, and recover the ethanol solution under reduced pressure to 1 / 5 of the original volume, add distilled water to 0.5 times the original volume, adjust the pH to 2, add the same volume of n-hexane or petroleum ether to extract twice, recover the organic solvent under reduced pressure to obtain saponified pine nut oil fatty acid;
[0023] B. Preparation of extract;
[0024] The preparation of the extract refers to the preparation of an aqueous solution containing inorganic ions and an organic phase containing pentaerythritol tetraoleate that can be combined with saturated fatty acids, wherein the inorganic ions can be comp...
Embodiment 2
[0034] Include the following steps:
[0035] A. the saponification treatment of pine nut oil;
[0036] Take the pine nut oil of Korean pine, put it in 80% ethanol solution of 6 times the volume, add sodium hydroxide or potassium hydroxide with a mass fraction of 20% of the ethanol solution, stir at room temperature for 36h or heat and reflux for 1.25h, and recover the ethanol solution under reduced pressure to 7 / 20 of the original volume, add distilled water to 1.25 times the original volume, adjust the pH to 2.5, add the same volume of n-hexane or petroleum ether to extract 3 times, recover the organic solvent under reduced pressure to obtain the saponified pine nut oil fatty acid;
[0037] B. Preparation of extract;
[0038] preparing copper sulfate 3mol / l inorganic ionic water, and adding methanol with a volume ratio of 1 / 5; the organic phase is petroleum ether containing pentaerythritol tetraoleate with a mass fraction of 10-30%;
[0039] C. complexed pinolenic acid;
...
Embodiment 3
[0047] Include the following steps:
[0048] A. the saponification treatment of pine nut oil;
[0049] Take the pine nut oil of Korean pine, place it in 100% ethanol solution of 8 times the volume, add sodium hydroxide or potassium hydroxide with a mass fraction of 30% of the ethanol solution, stir at room temperature for 48 hours or heat and reflux for 2 hours, and reclaim the ethanol solution under reduced pressure to the original 1 / 2 of the volume, add distilled water to double the original volume, adjust the pH to 3, add the same volume of n-hexane or petroleum ether to extract 3 times, recover the organic solvent under reduced pressure to obtain saponified pine nut oil fatty acid;
[0050] B. Preparation of extract;
[0051] preparing copper sulfate 4mol / l inorganic ionic aqueous liquid, and adding methanol with a volume ratio of 1 / 3; the organic phase is petroleum ether containing pentaerythritol tetraoleate with a mass fraction of 10-30%;
[0052] C. complexed pinolen...
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