LAMP and PCR non-diagnostic detection method for lawsonia intracellularis

Abstract

The invention relates to an LAMP and PCR non-diagnostic detection method for lawsonia intracellularis. Through online software, PCR and LAMP specific primers are designed aiming at a lawsonia intracellularis AM18025.2.1 gene sequence according to the primer design principle; and PCR and LAMP detection methods for the lawsonia intracellularis are successfully established after reaction condition optimization is carried out. Through comparison on a sensitivity test, a clinical sample detection test and a re-check detection test, the result shows that the sensitivity of the established LAMP method can reach 1.39*10<1> copies / mu l; the sensitivity of the established PCR method is only 1.39*10<4> copies / mu l; and the specificity and repeatability detection results show that the two methods have good specificity and relatively high repeatability, and show that the established PCR method and LAMP method are simple, convenient, fast and accurate and can be applied to clinical detection on the lawsonia intracellularis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a LAMP and PCR non-diagnostic detection method for Lawsonia intracellulare. Background technique [0002] Porcine proliferative enteropathy (PPE) is an acute infectious intestinal hemorrhagic wasting disease caused by the infection of Lawsonia intracellulare in pigs. The disease was first reported by Bieste and Sohwarce in 1931 and Rowland in 1974. It has been confirmed that the disease is one of the most widespread swine diseases in the world today. Although the mortality rate caused by infection with PPE is not high, because its main disease is chronic, the growth of sick pigs is slow, the average daily gain and feed utilization rate are significantly reduced, and the elimination rate increases, causing serious damage to pig farms. Economic losses. Porcine proliferative enteritis is a common disease in pigs all over the world in recent years. In countries and regions where the pi...

AI Technical Summary

Problems solved by technology

Among them, PCR is currently the most commonly used method for in vivo diagnosis of porcine proliferative enteritis. It has the characteristics of high sensitivity and good specificity, but its cost and requirements for the technology of operators and experimental equipment are relatively high.

Although Elanco's FIRST kit can quickly and accurately detect the presence of Lawsonia intracellulare in feces, it is expensive and not available in China

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