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Photosynthetic bacteria culture medium and preparation method thereof

A technology of photosynthetic bacteria and culture medium, applied in the direction of bacteria, etc., can solve the problems of long average enrichment time of culture medium, high cost, and many types of culture medium components, etc., achieve simple preparation process, simple composition, and reduce production costs Effect

Inactive Publication Date: 2015-12-30
RENMIN UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a photosynthetic bacteria culture medium with simple composition, which solves the technical problems of the existing photosynthetic bacteria culture medium, such as long average enrichment time of the existing photosynthetic bacteria culture medium, many types of culture medium components, and high cost.

Method used

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  • Photosynthetic bacteria culture medium and preparation method thereof
  • Photosynthetic bacteria culture medium and preparation method thereof
  • Photosynthetic bacteria culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, prepare photosynthetic bacteria culture medium

[0025] Weigh the following components: 6g sodium acetate trihydrate, 2g ammonium sulfate, 2g sodium bicarbonate, 0.4g potassium dihydrogen phosphate, 0.4g magnesium sulfate.

[0026] Prepare as follows:

[0027] Measure about 500ml of deionized water, accurately weigh 6g of sodium acetate trihydrate and add to water to dissolve, accurately weigh 2g of ammonium sulfate and add to water to dissolve, accurately weigh 2g of sodium bicarbonate and add to water to dissolve, accurately weigh 0.4g of potassium dihydrogen phosphate Add water to dissolve, accurately weigh 0.4g magnesium sulfate and add to water to dissolve. Then add deionized water to the solution to about 990ml, and stir evenly. The pH of the solution was adjusted to 7.5 using sodium hydroxide and hydrochloric acid. Finally, the solution was adjusted to 1 L.

[0028] The cost comparison of culture medium of the present invention and RCVBN culture...

Embodiment 2

[0037] Embodiment 2, prepare photosynthetic bacteria culture medium

[0038] Weigh the following components: Weigh the following components: 10g sodium acetate trihydrate, 5g ammonium sulfate, 5g sodium bicarbonate, 0.8g potassium dihydrogen phosphate, 0.8g magnesium sulfate.

[0039] Prepare as follows:

[0040] Measure about 500ml of deionized water, accurately weigh 10g of sodium acetate trihydrate and add to water to dissolve, accurately weigh 5g of ammonium sulfate and add to water to dissolve, accurately weigh 5g of sodium bicarbonate and add to water to dissolve, accurately weigh 0.8g of potassium dihydrogen phosphate Add water to dissolve, accurately weigh 0.8g of magnesium sulfate and add to water to dissolve. Then add deionized water to the solution to about 990ml, and stir evenly. The pH of the solution was adjusted to 7.5 using sodium hydroxide and hydrochloric acid. Finally, the solution was adjusted to 1 L.

[0041] The cost comparison of substratum of the pr...

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Abstract

The invention discloses a photosynthetic bacteria culture medium and a preparation method thereof. Each liter of the photosynthetic bacteria culture medium is prepared from the following components: 4g to 10g of sodium acetate trihydrate, 1g to 5g of ammonium sulfate, 1g to 5g of sodium hydrogen carbonate, 0.2g to 0.8g of monopotassium phosphate, 0.2g to 0.8g of magnesium sulfate and the balance of water. The pH value of the photosynthetic bacteria culture medium is 5.5 to 8.5. When the culture medium is prepared, the components are dissolved into the water, the pH value is regulated and then the volume is made to be constant. The photosynthetic bacteria culture medium provided by the invention has simple components and is only prepared from five types of common compounds; high-price substances including yeast extract, T.M storage liquid and the like in an existing culture medium are not used so that the production cost is reduced; compared with an RCVBN culture medium, the cost for producing 1kg of a bacterium culture medium can be saved by a maximum of 85%; the preparation process is simple and feasible and the operability is strong; by utilizing the culture medium to culture, the concentration of photosynthetic bacteria is high and the content of OD660 is up to 2.2.

Description

technical field [0001] The invention relates to a bacterial culture medium and a preparation method thereof, in particular to a photosynthetic bacteria culture medium and a preparation method thereof. Background technique [0002] Photosynthetic bacteria are a special group of microorganisms that can carry out photosynthesis without producing oxygen. They are widely distributed in oceans, rivers, lakes and soils, and can utilize organic matter, sulfide, Ammonia, etc. are used as hydrogen donors and carbon sources for photosynthesis. Photosynthetic bacteria are rich in proteins, biopolymers, antibiotics, carotenoids, pantothenic acid, and some medicinal substances. They are now widely used in aquaculture, planting, and sewage treatment Wait. Based on the above advantages, the promotion of photosynthetic bacteria should be increased. However, the medium used for cultivating photosynthetic bacteria has the disadvantages of long average enrichment time, various types of medium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 张光明彭猛王航瑶孟帆杨光王欣甜
Owner RENMIN UNIVERSITY OF CHINA
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