plac1 gene as a target for diagnosis and treatment of osteoarthritis

A PLAC1, osteoarthritis technology, applied in gene therapy, bone disease, disease diagnosis, etc., can solve problems such as early onset

Active Publication Date: 2019-03-01
BEIJING MEDINTELL BIOMED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, multiple studies have shown that genetic abnormalities can lead to early onset of OA

Method used

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  • plac1 gene as a target for diagnosis and treatment of osteoarthritis
  • plac1 gene as a target for diagnosis and treatment of osteoarthritis
  • plac1 gene as a target for diagnosis and treatment of osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Expression difference of PLAC1 gene in OA synovial tissue and normal synovial tissue

[0059] The synovial tissues of 8 OA patients were obtained from OA patients undergoing knee arthroplasty or synovectomy in the Department of Orthopedics of Peking Union Medical College Hospital. The cases used were in line with the diagnostic criteria for OA proposed by Altam. 8 cases of normal synovial tissues were from the Department of Orthopedics, Peking Union Medical College Hospital, which were joint synovial tissues of patients undergoing trauma surgery. The synovial fluid (SF) of patients with OA was centrifuged at high speed, and the supernatant was collected and stored at -80°C until use. All clinical samples used in this study were informed to the patients and approved by the Ethics Committee of our hospital.

[0060] 1. Extraction of total RNA from synovial tissue

[0061] The total RNA of the synovial tissue cells of OA patients and normal synovial tissue cel...

Embodiment 2

[0075] Example 2 Large sample verification of differentially expressed genes

[0076] The synovial tissues of 40 OA patients were obtained from OA patients undergoing knee replacement or synovectomy in the Department of Orthopedics of Peking Union Medical College Hospital. The cases used were in line with the diagnostic criteria for OA proposed by Altam. 40 cases of normal synovial tissues were obtained from the Department of Orthopedics of Peking Union Medical College Hospital, which were joint synovial tissues of patients undergoing trauma surgery. The synovial fluid (SF) of patients with OA was centrifuged at high speed, and the supernatant was collected and stored at -80°C until use. All clinical samples used in this study were informed to the patients and approved by the Ethics Committee of our hospital.

[0077] 1. Detection of differential expression of PLAC1 gene at the transcriptional level

[0078] 1.1 Extract synovial tissue total RNA

[0079] Step is with embod...

Embodiment 3

[0109] Example 3 OA fibroblast-like synoviocyte antibody neutralization experiment

[0110] 1. Steps

[0111] 1.1 In vitro culture of synovial tissue cells

[0112] After cleaning the aseptically obtained synovium tissue with PBS, cut it into tissue pieces of about 1mm x 1mm x 1mm repeatedly with sterile surgical scissors, add collagenase II (0.5mg / ml) at 37°C, digest for 2 hours, pass through 200 mesh Filter with gauze, centrifuge to remove supernatant, resuspend cells in DMEM medium, store at 37°C, 5% CO 2 Cultured in a cell culture incubator. After the cells grow into spindles and form sheets, they are subcultured. When the cells were passed to the third passage, FITC-labeled mouse anti-human CD3, CD14, CD19 and PE-labeled mouse anti-human CD11b were added for labeling, and flow cytometry was used for detection and identification. The cells that were negative for the above four markers were fibroblast-like synoviocytes (FLS) and were used in this study.

[0113] 1.3 An...

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Abstract

The invention discloses PLAC1 (placenta-specific 1) genes which can serve as a molecular marker for early diagnosis of osteoarthritis. Expressions of the PLAC1 genes in synovial tissue of a normal person and a patient suffering from osteoarthritis prove to be different in terms of the transcriptional level and the protein level, and accordingly, the disease risk and the disease state of a subject can be judged according to the identified expression level of the PLAC1 genes. Additionally, experiments prove that interference to expression of the PLAC1 genes or inhibition on the function of PLAC protein can inhibit proliferation of synovial cells, and a new target is provided for development of medicines for treating osteoarthritis clinically.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of human PLAC1 gene in the diagnosis and treatment of osteoarthritis. Background technique [0002] Osteoarthritis (OA) is a group of clinical syndromes with articular cartilage degeneration as the main pathological feature caused by various reasons. It is a common disease that mostly occurs after middle age. Progressive joint disease. Occurs mainly in articular cartilage. The main manifestations are the destruction of articular cartilage, loss of homogeneity, interruption, patch depression, fracture and ulceration of the cartilage surface. Some recent studies have shown that the occurrence of osteoarthritis is related to genetic factors. Synovium plays an important role in maintaining the normal function of joints and in the development of osteoarthritis. [0003] OA has a complex pathogenesis, and a variety of environmental and genetic factors lead to the occurrence and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K45/00A61K48/00A61K31/7088A61P19/02
CPCA61K31/7088A61K45/00C12Q1/6883C12Q2600/158G01N33/6893G01N2800/105
Inventor 董东孙耀兰杨承刚
Owner BEIJING MEDINTELL BIOMED CO LTD
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