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A kind of tissue culture propagation method of blood potato

The technology of an inoculation device and a connecting rod is applied in the fields of horticultural methods, botanical equipment and methods, horticulture, etc., which can solve the problems of reducing the reproduction coefficient of blood loose potato, cross-infection, low seed yield, etc., and improve the quality and reproduction of seedlings. coefficient, the effect of promoting differentiation and growth, and increasing the inoculation rate

Inactive Publication Date: 2018-06-19
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under natural conditions, blood-scattered potato mainly relies on seeds for breeding, but the seeds are small, and the characteristics of natural shedding when the seeds mature often result in low seed yields, making it difficult to achieve large-scale cultivation in production
[0004] Most of the traditional plant tissue culture inoculation loops are open loops fixedly connected to the rod body, which has serious cross-infection, greatly reduces the survival rate of inoculation, and reduces the reproduction coefficient of blood scattered potato. Therefore, there is an urgent need for a method to overcome bacterial cross-infection. , a plant tissue culture inoculation loop to improve the reproductive coefficient of blood-scattered potato

Method used

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  • A kind of tissue culture propagation method of blood potato
  • A kind of tissue culture propagation method of blood potato
  • A kind of tissue culture propagation method of blood potato

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Comparison scheme
Effect test

Embodiment 1

[0041] The blood scattered potato tissue culture propagation method of the present invention comprises the following steps:

[0042] (1) Acquisition of sterile explants: take bloody potato terminal buds as explants, place them in a beaker, soak them in 0.05% detergent solution by mass for 4 minutes, and gently clean the explants with a brush during the soaking process Surface dirt, then rinsed with running water for 10 minutes; transferred to the ultra-clean workbench, sterilized with 0.1% mercury liter added with 2 drops of Tween-20 for 8 minutes, then rinsed with sterile water for 3 times, and then rinsed with sterile water. Bacteria absorbent paper blots the surface moisture, cuts into 0.5cm stem segments with a terminal bud, and obtains aseptic explants; the sterile water is distilled water after autoclaving;

[0043] (2) Acquisition of sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into the induction medium, and cultivate under the condit...

Embodiment 2

[0048] The blood scattered potato tissue culture propagation method of the present invention comprises the following steps:

[0049] (1) Acquisition of sterile explants: take bloody potato terminal buds as explants, place them in a beaker, soak them in an aqueous solution of 0.05% detergent solution for 6 minutes, and gently clean the explants with a brush during the soaking process Surface dirt, then rinsed with running water for 20 minutes; transferred to the ultra-clean workbench, sterilized with 0.1% mercury liter added with 3 drops of Tween-20 for 10 minutes, then rinsed with sterile water for 5 times, and then rinsed with sterile water. Bacteria absorbent paper blots the surface moisture, cuts into 0.8cm stem segments with a terminal bud, and obtains aseptic explants; the sterile water is distilled water after autoclaving;

[0050] (2) Acquisition of sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into the induction medium, and cultivate ...

Embodiment 3

[0055] The blood scattered potato tissue culture propagation method of the present invention comprises the following steps:

[0056] (1) Acquisition of sterile explants: take bloody potato terminal buds as explants, place them in a beaker, soak them in an aqueous solution of 0.05% detergent solution for 5 minutes, and gently clean the explants with a brush during the soaking process Surface dirt, and then rinsed with running water for 15 minutes; transferred to the ultra-clean workbench, sterilized with 0.1% mercury liter added with 2.5 drops of Tween-20 for 9 minutes, then rinsed with sterile water for 4 times, and then rinsed with sterile water. Bacteria absorbent paper blots the surface moisture, cuts into a 0.6cm stem segment with a terminal bud, and obtains aseptic explants; the sterile water is distilled water after autoclaving;

[0057] (2) Acquisition of sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into the induction medium, and cult...

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Abstract

The invention discloses a method for tissue culture and propagation of blood scattered potato, which comprises the following steps: 1. acquisition of sterile explants; 2. acquisition of sterile test-tube seedlings; 3. propagation and cultivation of test-tube seedlings; Cultivation; 5. Test-tube seedling hardening and transplanting. The present invention adopts biotechnology to carry out tissue culture and propagation of the blood-scattered potato, maintains the excellent properties of the original variety, cultivates a large number of blood-scattered potato seedlings for field cultivation in a short period of time, and significantly improves the seedling quality and quality of the blood-scattered potato The reproduction coefficient is suitable for factory production, so as to meet the needs of production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for tissue culture and propagation of blood-scattered potato. Background technique [0002] Blood scattered potato, formal name used at school Stephania dielsiana Y.C.Wu, also known as gold does not change, a drop of blood, is a plant of the genus Stephaniaceae. Xuesan potato is used as medicine with tubers, cold in nature and bitter in taste; it has the effects of clearing heat and detoxifying, dispelling blood stasis and relieving pain, and is used for heatstroke fever, blood stasis and abdominal pain, coughing hemoptysis, lymphatic tuberculosis and other diseases. Blood-scattered potatoes are distributed in Guangxi, Hunan, Guangdong, Guizhou and other provinces and regions in my country, and are mainly produced in Guiping, Fangcheng, Yongning, Shanglin, Longlin, Tian'e, Nandan, Donglan, Luocheng, and Jinxiu in Guangxi. , Lipu, Yongfu, Lingchuan and other places. [0003]...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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