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A kind of method for transforming adventitious buds and rooting of soybean

A technology of bud rooting and soybean rooting, applied in the field of soybean transformation into adventitious bud rooting, can solve the problems of unfavorable physiological metabolism of regenerated seedlings, weak survival ability of rooted seedlings, and reduced survival rate of transplanting, so as to promote the occurrence of adventitious roots, improve rooting quality, and produce The effect of cumbersome process

Inactive Publication Date: 2017-12-15
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, it is necessary to prepare a solid rooting medium, which is complicated in technology and is easy to be infected with bacteria during the transfer of soybean adventitious buds to the medium and the subsequent cultivation process. The culture environment of the solid rooting medium is in a closed culture bottle. It is beneficial to the physiological metabolism of the growth of regenerated seedlings, inhibits the hairy roots of regenerated seedlings, and compared with the normal root system, the adventitious roots cultivated in the solid rooting medium have poor quality and no root hairs, and the absorption function is poor
The rooted seedlings cultivated in a closed environment in the bottle have weak survival ability, and usually have to go through the process of domestication outside the bottle, and then colonize. reduce

Method used

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  • A kind of method for transforming adventitious buds and rooting of soybean
  • A kind of method for transforming adventitious buds and rooting of soybean

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Experimental program
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Effect test

Embodiment 1

[0038] The method for this soybean conversion adventitious bud rooting that the embodiment of the present invention provides specifically comprises the following steps:

[0039] Soak the lower end of the transformed adventitious buds of soybeans in the rooting solution, then implant the soaked transformed adventitious buds into an unclosed seedling pot, cover with film, and carry out light cultivation. After new leaves grow, remove the film and continue to cultivate Until the root system is developed, the regenerated seedlings are strong.

Embodiment 2

[0041] The method for this soybean conversion adventitious bud rooting that the embodiment of the present invention provides specifically comprises the following steps:

[0042] S21, soak the lower end of the transformed adventitious buds of soybeans with a length greater than 3 cm in the rooting solution for 5 minutes;

[0043] Wherein, the composition of rooting liquid is as follows: indolebutyric acid 0.5mg / L, naphthaleneacetic acid 0.5mg / L, cephalosporin 200mg / L, pH=5.5;

[0044] S22. Put the seedling-raising substrate into the non-closed seedling-raising pot, water, and then implant the soaked soybean transformed adventitious buds in step S21 into the non-closed seedling-raising pot equipped with the seedling-raising substrate, and cover with a film;

[0045] S23. Perform light culture on the transformed adventitious buds of soybeans in step S22. The time of light culture is 14 hours / day, the intensity of light culture is 3000LX, and the temperature of light culture is 22...

Embodiment 3

[0048] The method for this soybean conversion adventitious bud rooting that the embodiment of the present invention provides specifically comprises the following steps:

[0049] S31, soaking the lower end of the soybean transformed adventitious buds with a length greater than 3 cm in the rooting liquid for 20 minutes;

[0050] Wherein, the composition of the rooting liquid is as follows: indolebutyric acid 1.0mg / L, naphthaleneacetic acid 0.2mg / L, cephalosporin 300mg / L, pH=6.0.

[0051] S32. Put the seedling-raising substrate into the non-closed seedling-raising pot, water, and then implant the soaked soybean transformed adventitious buds in step S31 into the non-closed seedling-raising pot equipped with the seedling-raising substrate, and cover with a film;

[0052] S33, transform the adventitious buds of soybeans in step S32 into light culture, the time of light culture is 15 hours / day, the intensity of light culture is 6000LX, the temperature of light culture is 23°C, open t...

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Abstract

The invention provides a method for rooting soybean transformed adventitious buds. The specific steps of the rooting method include: soaking the lower end of soybean transformed adventitious buds in rooting liquid, and implanting the soaked soybean transformed adventitious buds into an unclosed seedling-raising pot In the middle, cover the film and carry out light cultivation. After new leaves grow, remove the film and continue to cultivate until the seedlings are strong. The rooting solution used in the method for transforming adventitious buds into roots of soybeans provided by the invention has simple components and is easy to operate, and is not easy to breed Agrobacterium before the adventitious buds take root or after transplanting to the rooting culture medium, and the rooting rate can reach 96.24%. The method adopts direct rooting in an unclosed seedling pot (open environment), improves the quality of rooting, cultivates and obtains robust regenerated seedlings, thereby improving the transplanting survival rate of regenerated soybean seedlings, and the survival rate can reach 100%, and finally improves soybean transformation. Success rate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for transforming adventitious shoots into roots of soybeans. Background technique [0002] Soybeans are commonly known as soybeans, and soybeans are annual herbs in the leguminous family. Native to China, it is cultivated all over China and is also widely cultivated all over the world. Soybean is one of the important food crops in China. It is mainly produced in Northeast China. It is a crop whose seeds are rich in plant protein. Soybean is most commonly used to make various soy products, extract soybean oil, brew soy sauce and extract protein. Okara, or soybeans ground into a meal, are also commonly used in livestock feed. [0003] In the 1980s, Monsanto researchers cloned the resistance gene (EPsPs gene) from petunia, and introduced the EPsPs gene of petunia into the soybean genome by Agrobacterium-mediated method, and then bred the resistance gene (EPsPs gene) of glypho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 乔亚科董秋平乔潇李桂兰张锴
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY