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Integration reagent tube for luminescence immunodetection, and use method thereof

A technology of luminescent immunoassay and reagent tube, which is applied in the field of whole blood chemiluminescent immunoassay, can solve the problems of poor accuracy, repeatability, and low sensitivity, and achieve good repeatability, high sensitivity, and meet the effect of instant detection

Active Publication Date: 2016-01-13
XIAMEN XIANMING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The first object of the present invention is to provide an integrated reagent tube for luminescent immunoassay for instant detection in vitro diagnosis, to overcome the low sensitivity of existing instant detection (represented by colloidal gold and fluorescently labeled lateral immunochromatography technology) , poor accuracy and repeatability, so that the integrated reagent tube structure can detect whole blood and other samples for instant detection, and has the same sensitivity and accuracy as conventional diagnostic tools

Method used

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  • Integration reagent tube for luminescence immunodetection, and use method thereof
  • Integration reagent tube for luminescence immunodetection, and use method thereof
  • Integration reagent tube for luminescence immunodetection, and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one (The bottom surface of the microporous cup is integrally formed with a spherical segment as a reflector).

[0036] Such as Figure 1 to Figure 5 The shown integrated reagent tube for luminescence immunoassay includes a pre-solidified microporous cup 10 and a sample filter device 20 for filtering the liquid to be tested.

[0037] Such as figure 1As shown, the microporous cavity of the microporous cup 10 of the present invention is formed by connecting a round through hole 11 at the top and a cup hole 12 with a side ring wall 121 at the bottom to increase the upward projection and reflection focusing area. The cup of the present invention The hole is a cup hole 12 with a rounded table hole. The bottom surface 122 of the cup hole 12 is protrudingly provided with a reflector 30 that increases the upward projection and reflection focusing area, and a spherical segment 31 is used as a reflector (30 is integrated into the cup hole 12, the spherical segment 3...

Embodiment 2

[0048] Embodiment two (The integrally formed reflector on the bottom of the microporous cup can be replaced by other structures).

[0049] Such as Image 6 The difference between the shown integrated reagent tube for luminescence immunoassay and Example 1 is that the spherical segment 31 in Example 1 is replaced with one of cone 32, truncated cone 33, pyramid 34 or truncated prism 35; 32. The frustum 33, the pyramid 34 or the frustum 35 can increase the solid-liquid interface in the microhole cup 10, and the light on it can be reflected to the side wall of the cup hole 12 of the microhole cup 10 and converge after light detection. Make the photomultiplier tube (PMT) probe of the detection instrument receive more light signals from the solid-liquid interface; the sample filter device 20 of the integrated reagent tube for luminescent immunoassay in this embodiment is the same as that The method of using the integrated reagent tube for detection is the same as that in Example ...

Embodiment 3

[0050] Embodiment Three (Several tetrahedrons are integrally formed on the side ring wall 121 of the cup hole 12 of the microporous cup).

[0051] Such as Figure 7 As shown, the sample filtering device 20 of this embodiment is the same as that of Embodiment 1. A number of tetrahedrons 36 are integrally formed on the cup hole 12 side ring wall 121 of the microporous cup of this embodiment, and these tetrahedrons 36 increase the solid-liquid reaction Interface, and the solid-liquid reaction interface can be effectively irradiated by the projected light and reflected and converged, so that the light signal detected by the photomultiplier tube (PMT) probe of the detection instrument increases, and the detection sensitivity is greatly improved; the integrated reagent tube of this embodiment Using method is the same as embodiment one.

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Abstract

The invention discloses an integration reagent tube for luminescence immunodetection, and a use method thereof. The integration reagent tube comprises a micro-porous cup and a sample filtering device arranged at the cup mouth of the micro-porous cup, the sample filtering device comprises a socket piece in dismounting fit the cup mouth of the micro-porous cup, a plastic sample filtering cup fixed on the joint piece suspends at the top half in the micro-porous cup, and the bottom surface of the cup cavity of the sample filtering cup is fixedly provided with a binding film formed through laminating a sample filtering membrane and a marker film from top to bottom; and a material to be detected goes through the sample filtering device and undergoes an initial reaction, and the obtained material is added to the micro-porous cup provided with a solid phase in a dropwise manner and reacts to obtain a target reaction chain for luminescence immunodetection. Whole blood treatment, sample introduction, an immune reaction, washing, a luminescence reaction, and determination of a relative luminescence unit can be completed in the integration reagent tube without adding a reagent marker or the solid phase in the detection process, so the detection time is shortened; and the integration reagent tube is suitable for qualitative and quantitative instant detection of target immune molecules in whole blood, serum, blood plasma and other body fluids.

Description

technical field [0001] The invention relates to whole blood chemiluminescence immunoassay, in particular to an integrated reagent tube for luminescence immunoassay for instant detection in vitro diagnosis and its use method. Background technique [0002] Due to its high sensitivity, good stability and diverse and flexible operation on automated equipment, luminescent immunoassay technology occupies a core position in modern clinical immunological detection methods and quickly replaces radioimmunoassay technology; Bioactive substances or disease markers, as well as foreign antigens can be used to achieve micro-quantitative detection, which can be used for disease diagnosis, curative effect evaluation and prognosis judgment. [0003] Due to the multi-component characteristics of the reagent structure, the fully automatic detection system developed today involves multi-stage liquid transfer, and because multiple samples are run on the machine, the corresponding sample feeding a...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 徐恩良
Owner XIAMEN XIANMING BIOTECH CO LTD
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