Integration reagent tube for luminescence immunodetection, and use method thereof
A technology of luminescent immunoassay and reagent tube, which is applied in the field of whole blood chemiluminescent immunoassay, can solve the problems of poor accuracy, repeatability, and low sensitivity, and achieve good repeatability, high sensitivity, and meet the effect of instant detection
Active Publication Date: 2016-01-13
XIAMEN XIANMING BIOTECH CO LTD
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Abstract
The invention discloses an integration reagent tube for luminescence immunodetection, and a use method thereof. The integration reagent tube comprises a micro-porous cup and a sample filtering device arranged at the cup mouth of the micro-porous cup, the sample filtering device comprises a socket piece in dismounting fit the cup mouth of the micro-porous cup, a plastic sample filtering cup fixed on the joint piece suspends at the top half in the micro-porous cup, and the bottom surface of the cup cavity of the sample filtering cup is fixedly provided with a binding film formed through laminating a sample filtering membrane and a marker film from top to bottom; and a material to be detected goes through the sample filtering device and undergoes an initial reaction, and the obtained material is added to the micro-porous cup provided with a solid phase in a dropwise manner and reacts to obtain a target reaction chain for luminescence immunodetection. Whole blood treatment, sample introduction, an immune reaction, washing, a luminescence reaction, and determination of a relative luminescence unit can be completed in the integration reagent tube without adding a reagent marker or the solid phase in the detection process, so the detection time is shortened; and the integration reagent tube is suitable for qualitative and quantitative instant detection of target immune molecules in whole blood, serum, blood plasma and other body fluids.
Application Domain
Biological testing
Technology Topic
PhysicsChemistry +6
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