Applications of chitosanoligosaccharide in improvement of animal corpus luteum number, embryo nidation ability and fetal development ability
A technology of fetal development and corpus luteum number, applied in the application, animal feed, animal feed and other directions, to achieve the effect of improving the number of corpus luteum in the ovary, high physiological activity, and low dosage
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Embodiment example 1
[0017] Implementation case 1 Effect of chitosan oligosaccharides on reproductive performance such as the number of corpus luteum and the number of implanted embryos in the ovary of mice
[0018] (1) Experimental animals: female and male Kunming mice, clean animals, 3-4 weeks old, 12±3g, provided by the Experimental Animal Center of Dalian Medical University, approval number SCXK (Liao) 2008-0002, clean animals Raised in a room with a temperature of 20±1°C, a humidity of 40% to 50%, light for 12 hours, pellet feed, and free access to water. Feeding mice full-price compound feed for more than 4 weeks, the weight of mice can reach 26±3g, which can be used for mating experiments. The composition of the full-price compound feed is shown in Table 1.
[0019] (2) Experimental method: Female mice with similar body size and high energy were selected and randomly divided into two groups. The first group is the control group, fed with full-price compound feed without adding chitosan ol...
Embodiment example 2
[0059] Implementation case 2 Effect of chitosan oligosaccharides on the reproductive performance of mice in late pregnancy
[0060] (1) Experimental animals: female and male Kunming mice, clean animals, 3-4 weeks old, 12±3g, provided by the Experimental Animal Center of Dalian Medical University, approval number SCXK (Liao) 2008-0002, clean animals Raised in a room with a temperature of 20±1°C, a humidity of 40% to 50%, light for 12 hours, pellet feed, and free access to water. Feeding mice full-price compound feed for more than 4 weeks, the weight of mice can reach 26±3g, which can be used for mating experiments. The composition of the full-price compound feed is shown in Table 1.
[0061] (2) Experimental method: female mice with similar body size and high energy were selected and randomly divided into two groups. The first group is the control group, which is fed with full-price compound feed without chitosan oligosaccharide; the second group is chitosan oligosaccharide, ...
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