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Preparing method for serum-free and animal-source-free culture medium additive suitable for growth of insect cells

A technology of serum-free medium and insect cells, which is applied to animal cells, etc., can solve the problems of unsustainable maintenance of cell viability, difficulty in completely removing serum culture, and high price, so as to simplify the research and development process, shorten the doubling time, and promote rapid growth effect

Inactive Publication Date: 2016-01-20
内蒙古金源康生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the commercialized insect culture medium includes Grace medium, IPL-41 medium, and TC100 medium. These mediums are all classic mediums used in combination with serum, and there are also commercial serum-free medium such as: SF900II, SF900III , Express SFM, And other series of products, these products are expensive and the formula is confidential, and it is difficult to completely remove the serum culture, cannot maintain the viability of the cells continuously, or the cost is high, and there are few additives that replace the serum for insect cells.

Method used

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  • Preparing method for serum-free and animal-source-free culture medium additive suitable for growth of insect cells
  • Preparing method for serum-free and animal-source-free culture medium additive suitable for growth of insect cells
  • Preparing method for serum-free and animal-source-free culture medium additive suitable for growth of insect cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Configuration of serum-free medium suitable for insect cell growth

[0050] The present invention provides a serum-free medium suitable for insect cell growth and its preparation method. It uses Grace or IPL-41 produced by Gibico as the base medium to add amino acids, lipids, trace elements, hydrolyzates, etc., and the materials used are as follows: Special instructions are purchased from sigma company, the specific additives are as follows:

[0051] Glucose, 8g / L;

[0052] L-Arginine, 5.49mM;

[0053] L-Asparagine, 3.9mM;

[0054] L-Glycine, 12.89mM;

[0055] L-histidine, 2.77mM;

[0056] L-isoleucine, 8.41mM;

[0057] L-leucine, 10.4mM;

[0058] L-lysine, 7.59mM;

[0059] L-Methionine, 0.49mM;

[0060] L-threonine, 4.66mM;

[0061] L-tryptophan, 0.91mM;

[0062] L-Jetine, 4.52mM;

[0063] L-proline, 8.22mM;

[0064] Cottonseed hydrolyzate 800mg / L;

[0065] Rice hydrolyzate 3000mg / L;

[0066] Wheat bran hydrolyzate 300mg / L;

[0067] Recombinant ...

Embodiment 2

[0088] Embodiment 2. The contrast experiment that culture medium of the present invention is used for cell culture contrast experiment is divided into two groups:

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Abstract

The invention relates to the technical field of biological pharmacy, in particular to development of a serum-free and animal-source-free culture medium additive suitable for insect cells mainly including Sf9 and High Five cells. A traditional culture medium Grace or IPL-41 serves as a basis, the additive beneficial for the growth of the insect cells is added, in the insect cell culture process, the additive has a function of replacing serum, price is low, the insect cells can grow for 240 h continuously, and cell activity can keep over 95%.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the development of an additive suitable for the serum-free and animal-source-free cultivation of insect cells (mainly including Sf9 and HighFive cells). Background technique [0002] At present, commonly used insect cells include Sf-9 cells, namely: Spodopterafrugiperda (Spodoptera frugiperda) cells and HighFive cells, and HighFive cells (BTI-TN-5B1-4) are derived from the parental cell line of Trichopulsiani The clonal isolates are commonly used for the expression of recombinant proteins in the baculovirus expression vector system (BEVS). The baculovirus expression system (baculovirus expression system) is a eukaryotic expression system that has been widely used in recent years. It can express a variety of foreign genes in insect cells, including genes of fungi, plants, bacteria, and viruses. Baculovirus is a kind of double-stranded DNA virus with insect cells as nat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07
Inventor 赵峻岭苏广华黄新朋
Owner 内蒙古金源康生物工程股份有限公司
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