Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of pecan mir166d in advancing plant flowering or plant meristem development

A mir166d, meristem technology, applied in angiosperms/flowering plants, applications, plant products, etc., can solve problems such as the reduction of the number of root nodules and lateral roots, and abnormal development of the vascular system.

Inactive Publication Date: 2018-03-30
ZHEJIANG FORESTRY UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overexpression of miR166 in Medicago truncatula can reduce the number of nodules and lateral roots, and abnormal development of vascular system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of pecan mir166d in advancing plant flowering or plant meristem development
  • Application of pecan mir166d in advancing plant flowering or plant meristem development
  • Application of pecan mir166d in advancing plant flowering or plant meristem development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] 1. Materials

[0020] 1.1 Experimental materials

[0021] Hickory flower buds were collected from Banqiao Village, Lin'an, Hangzhou City, Zhejiang Province, and different hickory trees were selected for sampling. Immediately after the samples were collected, they were stored in liquid nitrogen, brought back to the laboratory and stored in a -80°C refrigerator for later use. In addition, Arabidopsis thaliana mutant seeds transgenic for miR166d were purchased (purchased from ABRC, USA).

[0022] 1.2 Experimental reagents and instruments

[0023] DNA polymerase, various restriction enzymes, T4 ligase, Marker and TRIzol reagents were purchased from Bao Biological Engineering (Dalian) Co., Ltd.; plasmid extraction kit and DNA gel recovery kit were purchased from Shanghai Sangon Bioengineering Co., Ltd. Ltd. The PCR instrument was a PE9700 PCR instrument from the United States, and the ultra-clean workbench was purchased from Suzhou Chengjing Purification Technology Co., ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of pecan miR166d in advancing plant flowering or plant meristem development. The application of pecan miR166d is expected to regulate the flowering and meristem development of plants.

Description

technical field [0001] The present invention relates to the field of gene function, and more specifically relates to the application of pecan miR166d in advancing plant flowering or plant meristem development. Background technique [0002] MicroRNA (miRNA) is a class of non-coding small RNA molecules (18-25 nucleotides) that are widely present in animals and plants. They contain stem-loop structure miRNA precursors and are processed by Dicer. miRNA regulates gene expression by inhibiting the translation or cleaving the target gene of the target gene on the genome at the transcriptional or post-transcriptional level, and negatively regulates its target gene in gene expression. miRNAs play a key role in the entire floral development process of plants from flower induction to the formation of floral organ characteristics. [0003] Hickory (Carya cathayensis Sarg.) belongs to the Juglandaceae Hickory plant, monoecious, mainly distributed in western Zhejiang and Anhui, is a uniq...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00A01H6/20
Inventor 王正加黄坚钦孙志超
Owner ZHEJIANG FORESTRY UNIVERSITY