Method for synchronously preparing sardine oil and umami peptide

Abstract

The invention provides a method for synchronously preparing sardine oil and a umami peptide and belongs to the techniques of extracting and processing of oil of aquatic products. The method comprises steps as follows: (1), fresh sardine meat is minced and mixed with water, and a mixed solution is obtained and subjected to ultrasonic treatment; (2), compound protease is added to the mixed solution after ultrasonic treatment for first time of enzymolysis, enzyme deactivation and centrifugal separation are performed after enzymolysis, a liquid phase is obtained, the pH (potential of hydrogen) of the liquid phase is adjusted to be 4.5, then filtering is performed under reduced pressure, and filter liquor and filter residues are obtained; (3), the filter liquor is demulsified through high-pressure steam and subjected to centrifugal separation after demulsification, and sardine oil is obtained; (4), water is added to the filter residues, the filter residues are redissolved, flavored proteinase is added for second time of enzymolysis, enzyme deactivation and centrifugal separation are performed after enzymolysis, supernatant is obtained and subjected to vacuum concentration, spraying drying is performed, and then the umami peptide is obtained. An ultrasonic treatment and separate enzymolysis combination method is adopted for preparing the sardine oil and the umami peptide, the prepared sardine oil and the umami peptide have high nutritional value and good quality, and the method is safe, pollution-free and suitable for the field of food processing.

Description

technical field [0001] The invention belongs to the oil extraction and processing technology of aquatic products, and mainly relates to a method for synchronously preparing sardine oil and umami peptide. Background technique [0002] Sardine (Sardinapilch-ardus) is a fish with high nutritional value, rich in phospholipids, namely OMEGA-3 fatty acids, protein and calcium. The traditional methods of extracting sardine oil are pressing and solvent extraction. Among them, after oil extraction by pressing method, after cooking the raw materials, mechanical pressure is used to destroy the combination of protein and fat, and then the crude fish oil is obtained by centrifugation. This method is simple but the fish oil extraction rate is low, the product quality is poor, and the energy consumption is high, and the parts are easy to wear and tear. The solvent method is to dissolve fish oil in a solvent, then separate the solvent phase, and recover the solvent to obtain fish oil. The...

AI Technical Summary

Problems solved by technology

This method is simple but the extraction rate of fish oil is low, the product quality is poor, and the energy consumption is high, and the parts are easy to wear out

The solvent method is to dissolve fish oil in a solvent, then separate the solvent phase, and recover the solvent to obtain fish oil. The process requires a large amount of solvent, and at the same time, the residual solvent in the obtained fish oil product is difficult to be completely removed.

[0003] In the invention of application number 201410269903.X "a sardine hypotensive peptide and its preparation method and application", papain and alkaline protease are used for enzymatic hydrolysis, and then ultrafiltration and gel chromatography are used to separate and purify the crude product ; Freeze-dry the high-efficiency components after separation and purification to obtain sardine blood pressure-lowering peptides. Although sardine peptides can be prepared by enzymatic methods, this method is complicated to operate and the process is cumbersome, and it will affect the extraction rate of sardine oil