Method for converting ginsenoside through plant complex enzyme

A technology for transforming human and ginsenosides with compound enzymes is applied in the field of utilizing plant compound enzymes to transform ginsenosides, which can solve the problems of poor specificity, low yield, environmental pollution and the like, and achieve the effects of easy product drying, convenient operation and simple process.

Inactive Publication Date: 2016-01-20
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical methods such as acid hydrolysis, alkali hydrolysis and Smith degradation method, etc., although the operation is simple, but the specificity is poor, it is difficult to obtain a monomeric saponin with a specific structure, and the yield is very low, causing environmental pollution at the same time, not suitable for industrial production

Method used

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  • Method for converting ginsenoside through plant complex enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Configure LL liquid medium (0.5gNH 4 Cl, 1.0gK 2 HPO 4 , 0.5gKH 2 PO 4 , 0.25gMgSO 4 and 1.0g yeast powder, water 1000ml, pH value 7.0), sterilized at 121°C for 15min, cooled to room temperature; Ginseng was ground into powder, sterilized at low temperature at 50-90°C, then cooled to room temperature; 4.8g Mix the sterilized ginseng powder with 1.2g plant compound enzyme; add LL liquid medium and distilled water to adjust the moisture, the moisture in the solid fermentation system is 50-80%; mix well, seal it, and place it in a constant temperature incubator at 30°C Ferment for 7 days to obtain ginseng fermentation product; use 50-200mL of 80% methanol aqueous solution for the fermentation product, ultrasonically extract at 50°C for 10-50min, filter, and concentrate the filtrate under reduced pressure to obtain the crude ginsenoside extract; add the crude ginsenoside extract to After dissolving in ionic water, extract with saturated n-butanol solution, concentrate ...

Embodiment 2

[0023] Configure LL liquid medium (0.5gNH 4 Cl, 1.0gK 2 HPO 4 , 0.5gKH 2 PO 4 , 0.25gMgSO 4 and 1.0g yeast powder, water 1000ml, pH value 7.0), sterilized at 121°C for 15min, cooled to room temperature; Ginseng was ground into powder, sterilized at low temperature at 50-90°C, then cooled to room temperature; 4.8g Mix the sterilized ginseng powder with 1.2g plant compound enzyme; add LL liquid medium and distilled water to adjust the moisture, the moisture in the solid fermentation system is 50-80%;; mix well, seal it, and place it in a 40°C constant temperature incubator The ginseng fermentation product was obtained by medium fermentation for 8 days; the fermentation product was extracted with 50-200mL of 80% methanol aqueous solution, ultrasonically extracted at 50°C for 10-50min, filtered, and the filtrate was concentrated under reduced pressure; extraction and concentration under reduced pressure to obtain solid fermented ginsenoside extract. According to the results ...

Embodiment 3

[0025] Configure LL liquid medium (0.5gNH 4 Cl, 1.0gK 2 HPO 4 , 0.5gKH 2 PO 4 , 0.25gMgSO 4 and 1.0g yeast powder, water 1000ml, pH value 7.0), sterilized at 121°C for 15min, cooled to room temperature; Ginseng was ground into powder, sterilized at low temperature at 50-90°C, then cooled to room temperature; 3.6g Mix the sterilized ginseng powder with 2.4g plant compound enzyme; add LL liquid medium and distilled water to adjust the moisture, the moisture in the solid fermentation system is 50-80%; mix well, seal it, and place it in a constant temperature incubator at 50°C Ferment for 9 days to obtain ginseng fermentation product; use 50-200mL of 80% methanol aqueous solution for the fermentation product, ultrasonically extract at 50°C for 10-50min, filter, and concentrate the filtrate under reduced pressure; add deionized water to dissolve, then extract with saturated n-butanol solution , concentrated under reduced pressure to obtain solid fermented ginsenoside extract. ...

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Abstract

The invention discloses a method for converting ginsenoside through plant complex enzyme. The method comprises the following steps that ginseng is ground into powder, subjected to low-temperature sterilization and then cooled to the room temperature; the sterilized ginseng powder is evenly mixed with the plant complex enzyme, moisture is adjusted through a sterile LL liquid culture medium and distilled water, the mixture is fully mixed to be uniform, then sealed and put into a constant-temperature incubator for fermentation, and a ginseng fermentation product is obtained; an 80% methyl alcohol aqueous solution is used for ultrasonic extraction, filtering is carried out, filtrate is subjected to vacuum concentration, and coarse ginsenoside extract is obtained; the coarse ginsenoside extract is dissolved by adding deionized water, then subjected to extraction through a saturate n-butyl alcohol solution and subjected to vacuum concentration, and ginsenoside extract is obtained, wherein the ginsenoside extract contains rare ginsenoside with fewer sugar chains or an antineoplastic active material protopanoxadiol (PPD) without sugar chains. The method is simple in process and convenient to implement, and the product is easy to dry; most importantly, in the solid fermentation process, conversion of ginsenoside is achieved, and the rare ginsenoside or the antineoplastic active material protopanoxadiol (PPD) is obtained.

Description

technical field [0001] The invention belongs to the field of biotransformation of natural products, and in particular relates to a method for transforming ginsenosides by using plant complex enzymes. Background technique [0002] Ginseng is a plant of the genus Panaxginseng C.A.Mayer of the family Araliaceae. It is called ginseng because its roots are hypertrophic, with forks, and its shape looks like a human. The dried roots and rhizomes of ginseng have the functions of invigorating the spleen and lungs, eliminating evil spirits, invigorating vitality, tranquilizing the spirit, relieving panic and palpitations, improving eyesight and improving intelligence. one. The first pharmacy classic in the history of our country - "Shen Nong's Materia Medica", ginseng is called "the main nourishing life to respond to the sky, non-toxic, long-term use will not hurt people, light body, nourishing qi, not aging and prolonging life" "One of the best medicines. Ginseng is mainly distrib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12P33/00
Inventor 尹成日陈贺尹振浩宋晓琳金英今
Owner YANBIAN UNIV
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