A kind of potassium decomposing microorganism and its application in crop planting
A microbial preparation and potassium decomposing technology, applied in the field of microbiology, can solve problems such as ineffective potassium decomposing effect, unstable production increase effect, and weak strain survival ability, so as to achieve good soil improvement effect, benefit crop seedling cultivation and increase production stable effect
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Embodiment 1
[0030] 1. Screening and identification of Bacillus glialis NGW1
[0031] A large number of strains were screened from the soil. Select a single colony through a screening plate, and use an atomic absorption instrument to detect potassium in the fermentation supernatant to obtain the NGW1 strain. The specific screening method is as follows: take the potassium-dissolving bacteria isolation plate as the screening condition, and initially isolate the bacteria from the soil by the dilution method. For colonies with smooth edges, translucent, shiny, viscous colloids, and larger diameters, continue to streak culture until a single colony is obtained; activate the single colony and insert it into the re-screening medium of potassium-dissolving bacteria for 7 days, and use an atomic absorption instrument Potassium content in the fermentation supernatant was detected to obtain high-efficiency potassium-decomposing bacteria NGW1.
[0032] After such figure 1According to the phylogeneti...
Embodiment 2
[0081] Embodiment 2 is basically the same as Embodiment 1, and the effect is close, the difference is:
[0082] One, the present embodiment makes the potassium-decomposing microbial preparation with the fermentation liquid of bacillus colloidus NGW1: the volume ratio of wheat bran=1:2, and concrete steps comprise:
[0083] Activation: Inoculate Bacillus colioids NGW1 in the preservation medium for activation, and culture it in a constant temperature incubator at 30°C for 20 hours. The composition of the above preservation medium is: 5% sucrose, 0.5% dipotassium hydrogen phosphate, 0.5% magnesium sulfate, 0.5% calcium carbonate, 1.5% agar, pH 7.5, sterilized at 115°C for 20 minutes before use.
[0084] Seed liquid preparation: Pick a single colony of activated Bacillus colioids NGW1 on the plate and inoculate it into the liquid seed medium, and culture it at 25°C for 10 hours to reach the cell concentration OD 600 When it reaches 0.5-0.6, the strain seed liquid is obtained. T...
Embodiment 3
[0093] Embodiment 3 is basically the same as Embodiment 1, and the effect is close, the difference is:
[0094] In this embodiment, the potassium-dissolving microbial preparation is made with the fermentation broth of Bacillus colloidus NGW1: wheat bran=1:10 by volume, and the specific steps include:
[0095] Activation: Inoculate Bacillus colioids NGW1 into the preservation medium for activation, and culture it in a constant temperature incubator at 35°C for 15 hours. The composition of the above preservation medium is: 3% sucrose, 1% dipotassium hydrogen phosphate, 1% magnesium sulfate, 1% calcium carbonate, 1.8% agar, pH 7.5, sterilized at 115°C for 20 minutes before use.
[0096] Seed liquid preparation: Pick a single colony of activated Bacillus colioids NGW1 on the plate and inoculate it into the liquid seed medium, and culture it at 35°C for 9 hours to reach the cell concentration OD 600 When it reaches 0.5-0.6, the strain seed liquid is obtained. The composition of t...
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