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Simple production method of high-content galactooligosaccharides

The technology of a galacto-oligosaccharide and a production method is applied in the field of preparation of galacto-oligosaccharide, which can solve the problems of restricting the entry of galacto-oligosaccharide, increasing the cost, high price and the like, and achieving the effect of low equipment price

Inactive Publication Date: 2016-01-27
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of this method requires the purchase of expensive simulated moving bed chromatographic separation equipment, which increases the cost of production and limits the entry of the galactooligosaccharide industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Dissolve 100 grams of lactose in water, adjust to a concentration of 45%, and keep warm at 95-100°C for 20 minutes. Add β-galactosidase according to the enzyme amount of 50u / g* lactose, adjust the pH to 5.5-6.0, and react at 40°C. Feed 100g of lactose, adjust the lactose concentration to 70%, and the temperature is 55-90°C, then add to the reaction system and add while stirring. After the addition, adjust the pH to 5.5-6.0, and continue the reaction at 40°C for 100h. Add 4 grams of activated carbon to raise the temperature to 90°C and keep it warm for 30 minutes. Activated carbon is removed by suction filtration, and the conductance of the feed liquid is reduced by ion exchange resin. The separation requirement is: concentration 30%, conductance 0-100us / cm. Concentrate the feed liquid to 75% to obtain a galacto-oligosaccharide syrup with a concentration of 75% and a content greater than 57%.

Embodiment 2

[0037] Dissolve 1 ton of lactose in water, adjust to a concentration of 40%, and keep warm at 95-100°C for 20 minutes. Add β-galactosidase according to the enzyme amount of 100u / g* lactose, adjust the pH to 5.5-7.0, react at 45°C for 20 hours, and then start feeding high-concentration lactose solution. Adjust 10 tons of lactose to 70%, and keep warm at 95-100°C for 20 minutes. According to the flow rate of 100L / h flow into the reaction system. Add 40Kg of activated carbon to raise the temperature to 70-80°C and keep it warm for 30min. Activated carbon is removed by plate and frame filter, and the conductance of the feed liquid is reduced by ion exchange resin. The separation requirement is: concentration 30%, conductance 0-100us / cm. Use a multi-effect evaporator to concentrate the feed liquid to 70% in vacuum to obtain a galacto-oligosaccharide syrup with a concentration of 70% and a content greater than 57%.

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Abstract

The invention provides a simple production method of high-content galactooligosaccharides, comprising following steps: lactose dissolution, enzymatic reaction, decoloring by enzyme deactivation, desalination by nano-filtration, ion exchange, concentration, and preparation of high-purity galactooligosaccharide syrup. By the adoption of the method in producing high-purity galactooligosaccharide syrup, reaction system sugar concentration can be improved, lactose crystal is avoided occurring in the production process, and the component of galactooligosaccharides may reach higher than 57%; the component of galactooligosaccharides produced according to the method meets the requirements of related national standards, and industrial production is facilitated.

Description

technical field [0001] The invention relates to a method for preparing oligosaccharides, in particular to a method for preparing galactooligosaccharides. Background technique [0002] Galacto-oligosaccharide (GOS) is a functional oligosaccharide, which is an oligosaccharide with 1-4 galactosyl groups attached to the galactosyl side of the lactose molecule. Naturally occurring galacto-oligosaccharides are derived from animal milk. Galacto-oligosaccharides are the proliferation factors of bifidobacteria in the intestines, and the establishment of intestinal flora in infants and young children largely depends on the galacto-oligosaccharides components in breast milk. In addition, galactooligosaccharides can also improve fat metabolism and promote calcium absorption. Widely used in baked food, milk products for infants and young children, anti-caries food, etc. As a new resource food, galacto-oligosaccharides are required to contain more than 57%. [0003] β-Galactosidase is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12N9/38C07H3/06C07H1/06C12R1/07
Inventor 刘宗利王乃强刘峰李克文杨业坤栾庆民郑传宝冉金宝李燕燕王保忠
Owner BAOLINGBAO BIOLOGY
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