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CYP4F2*3 detection primer and detection system thereof

A technology for detecting primers and systems, applied in the fields of molecular biology and nucleic acid detection, can solve the problems of high cost, complicated operation, long test time, etc., and achieve the effects of simple operation, low detection cost, and short test time.

Inactive Publication Date: 2016-01-27
BEIJING JINQI BIOLOGICAL TECH CO LTD
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Problems solved by technology

[0007] The problem to be solved by the present invention is that the existing methods for detecting the CYP4F2 genotype have high cost, complicated operation and long testing time

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  • CYP4F2*3 detection primer and detection system thereof
  • CYP4F2*3 detection primer and detection system thereof
  • CYP4F2*3 detection primer and detection system thereof

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Embodiment Construction

[0034] In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0035] A detection primer for CYP4F2*3, which includes the following primers:

[0036] 5'-ATCAACCCGTTCCCACCTCAGAC-3' (SEQ ID No.1, hereinafter referred to as P)

[0037] 5'-CAAAGGTGCTCACAGCCTCACG-3' (SEQ ID No.2, hereinafter referred to as Q)

[0038] 5'-ACCTCAGGGTCCGGCCAAAC-3' (SEQ ID No.3, hereinafter referred to as F)

[0039] 5'-CGGAACCCATCACAACCCAGATA-3' (SEQ ID No. 4, hereinafter referred to as R).

[0040] A CYP4F2*3 detection system includes the above primers, buffer, Taq enzyme and dNTP. The concentration of each component in the detection system can be carried out according to the existing concentration required for PCR. As a preferred solution, in this embodiment, the concentration of each primer is 200uM, the concentration of dNTP is 100uM, and the concentration of Taq enzyme is 5...

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Abstract

The invention provides a CYP4F2*3 detection primer and a detection system thereof, and belongs to the technical field of molecular biology and nucleic acid detection. The CYP4F2 genotype primer and the system are designed by employing a bidirectional amplification refractory mutation system PCR (Bi-Amplification Refractory Mutation System PCR, BI-ARMS-PCR) technology on the basis of ARMS-PCR. A specific primer is designed aiming at two alleles of rs2108622, and the rs2108622 site base in a to-be-detected sample is determined by reading the electrophoresis band quantity and size, and thus whether a tested person carries CYP4F2*3 is determined, and the tested person is guided for medicine usage.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and nucleic acid detection, in particular to a CYP4F2*3 detection primer and a detection system thereof. Background technique [0002] Warfarin is a coumarin-type anticoagulant. As one of the most widely used anticoagulant drugs in clinical practice, it has the advantages of low price and effective effect in small doses. It is mainly used to prevent and treat venous arterial thromboembolic diseases , the applicable patient population is patients with heart valve replacement, atrial fibrillation, deep vein thrombosis and pulmonary embolism. However, currently clinically, the administration of warfarin faces a serious problem, that is, the dosage of warfarin is very difficult to control, an insufficient dosage will lead to the generation of thrombus in the body, and a slightly larger dosage will increase the tendency of bleeding. [0003] At present, clinical workers at home and abroad u...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q2525/185C12Q2531/113C12Q2535/137C12Q2565/125
Inventor 汪大为
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD
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