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Application of aliphatic acid compound for promoting denitrification activity of denitrifying microorganism

A technology for denitrifying microorganisms and fatty acids is applied in the application field of fatty acid compounds in promoting the denitrification activity of denitrifying microorganisms. The effect of promoting activity, preventing water quality deterioration, and being less prone to loss

Inactive Publication Date: 2016-02-03
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The substance can improve the nitrogen removal efficiency of water by promoting the activity of denitrifying microorganisms, strengthen the plant-microbe rhizosphere effect, and solve the technical problem of low nitrogen removal efficiency when dealing with low C / N farmland drainage in water pollution control in a simple way At the same time, the substance of the present invention can also improve the problem of easy loss of external carbon sources and decomposition by microorganisms, improve the denitrification efficiency of denitrifying microorganisms on farmland drainage, and save the cost of external carbon sources

Method used

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  • Application of aliphatic acid compound for promoting denitrification activity of denitrifying microorganism
  • Application of aliphatic acid compound for promoting denitrification activity of denitrifying microorganism
  • Application of aliphatic acid compound for promoting denitrification activity of denitrifying microorganism

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 The effect comparison between fatty acid compounds and similar substances that efficiently promote nitrogen removal

[0023] 1.1 Experimental design

[0024] (1) Standard products: 5 fatty acid compounds, palmitic acid, stearic acid, arachidic acid, behenic acid and linoleic acid, all purchased from Sigma- Aldrich Company (St.Louis, MO, USA), and dissolved in CH 2 Cl 2 middle.

[0025] (2) Microbial strain: Pseudomonas fluorescens (ACCC01047), purchased from China Agricultural Microorganism Culture Collection Management Center.

[0026] (3) LB medium: 10g / Ltryptone, 5g / Lyeastextract, 10g / LNaCl, pH7.0.

[0027] (4) Denitrification medium: 0.72g / LKNO 3 , 1.0g / LKH 2 PO 4 , 0.20g / LMgSO 4 ·7H 2 O, 2.8g / LC 4 h 4 Na 2 o 4 ·6H 2 O, pH7.0.

[0028] (5) Biological activity experiment

[0029] Pseudomonas fluorescens activated in LB medium for 20 h were collected, centrifuged for 10 min (5,000 g, 4° C.), and then resuspended in sterile denitrification med...

Embodiment 2

[0041] The promoting effect of embodiment 2 accelerator linoleic acid on Pseudomonas fluorescens denitrification efficiency

[0042] 2.1 Experimental design

[0043] Add 5, 10, 20, 40, 60, 80mg·L respectively -1 The accelerator linoleic acid of the present invention is added to the denitrification culture solution, and then 1 mL of Pseudomonas fluorescens bacteria solution is added, and cultivated in a shaker at 30 ° C and 120 rpm, and the control is CH 2 Cl 2 . After culturing for 72 hours, the bacterial solution was centrifuged according to the method in 1.1, and the total nitrogen concentration was measured in the supernatant, and the denitrification promotion rate (%) after each treatment was calculated.

[0044] 2.2 Experimental results

[0045] The accelerator of the present invention is added at a concentration of 5-80mg L -1 When, the denitrification rate of Pseudomonas fluorescens has about 10-50% promotion effect ( image 3 ). Due to the addition of 60mg·L -1...

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Abstract

The invention relates to an application of an aliphatic acid compound for promoting denitrification activity of denitrifying microorganism, which provides the application of the compound with a structural formula in a specification for promoting the denitrification activity of the denitrifying microorganism. The compound is separated from a water plant duckweed extract product, has good nitrogen element-removal promotion activity, with low dosage of the compound, nitrogen removal efficiency of common denitrifying bacterium pseudomonas fluorescens can be raised effectively. The compound can be used as a purifying synergist of a nitrate-polluted water body in the field of agricultural environmental protection.

Description

technical field [0001] The invention belongs to the research field of control technology of nitrate-polluted water bodies, and in particular relates to the application of a fatty acid compound in promoting denitrification activity of denitrifying microorganisms. Background technique [0002] The first national survey of pollution sources in 2010 showed that nitrate pollution from agricultural sources has surpassed industrial sources to become the main source of total nitrogen pollution in water bodies in my country, causing eutrophication of rivers and lakes, leading to surface water pollution and excessive nitrate in groundwater. , a serious threat to human health. From a long-term perspective, excessive nitrate will lead to changes in the structure and diversity of the entire ecological community. Therefore, the purification treatment of agricultural wastewater and fertilizer water has become a new research hotspot in the field of water treatment. Using aquatic plants wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34
Inventor 陆玉芳施卫明孙力
Owner INST OF SOIL SCI CHINESE ACAD OF SCI