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Microfluidic SERS chip detection device integrated with liquid core optical waveguide and nanometal

A liquid-core optical waveguide and nano-metal technology, applied in the direction of material excitation analysis, Raman scattering, etc., can solve the problem of fewer types and methods of liquid-core optical waveguide, improve sensitivity and reproducibility, small size, and easy to carry Effect

Active Publication Date: 2016-02-03
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few types and methods of liquid-core optical waveguides integrated in microchannels, and the in-situ integration of nano-metal structures on the inner surface of liquid-core optical waveguides has not been reported.

Method used

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  • Microfluidic SERS chip detection device integrated with liquid core optical waveguide and nanometal
  • Microfluidic SERS chip detection device integrated with liquid core optical waveguide and nanometal
  • Microfluidic SERS chip detection device integrated with liquid core optical waveguide and nanometal

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Effect test

Embodiment 1

[0019] The specific implementation steps of the integrated preparation of TeflonAF1600 liquid core optical waveguide and nano gold in the microchannel detection area:

[0020] (1) Prepare a substrate with microchannels, and tightly combine the substrate with the PDMS cover to form a closed microchannel structure.

[0021] (2) Use 1H, 1H, 2H, 2H-perfluorodecyltriethoxysilane to increase the binding degree of TeflonAF1600 to the microchannel; use physical deposition to wrap TeflonAF1600 into the detection area of ​​the microchannel, and integrate liquid in the detection area Core optical waveguide structure;

[0022] (3) Nano-gold is assembled on the surface of TeflonAF1600 inside the waveguide by chemical self-assembly method, and an integrated SERS detection microstructure of liquid-core optical waveguide and nano-gold is formed in the detection area.

[0023] In this embodiment, step (2) includes pretreatment of the PDMS substrate, modification of 1H, 1H, 2H, 2H-perfluorodec...

Embodiment 2

[0036] The SERS application detection was performed on the SERS micro-detector in Example 1. Serum and biological samples are passed into the SERS detector of the microfluidic chip 9 by pressure injection, and then the prefabricated support 11 is placed on the stage 10, and then the microfluidic chip is fixed on the support. Using a LabRAMHREvolution Raman spectrometer (HORIBA Jobin Yvon S.A.S., France), move the bracket so that the microscope objective lens 7 is aligned with the exit end D of the microchannel, so that both the excitation light and the Raman light 8 are coupled to the objective lens from the same exit end D of the detection area . The wavelength of the selected laser is 633nm, the laser power is 17mW, the attenuation film is 20%, the number of exposures is 2 times, the integration time is 2s, and the detection volume is 1ul for SERS activity test to obtain the SERS spectrum of human serum.

[0037] For the SERS spectrum of human serum, see Figure 4 , and th...

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Abstract

The invention provides a microfluidic SERS chip detection device integrated with liquid core optical waveguide and nanometal, a chip preparation method and a detection method. A chip is assembled by a base plate and a cover plate, wherein a micro channel is formed in the chip; a sample inlet and a sample outlet are formed in two ends of the micro channel; a detection area is arranged in the micro channel; Teflon AF liquid core optical waveguide is integrated in the detection area; a nanometal structure is fixed on the inner surface of the Teflon AF liquid core optical waveguide. Through adoption of synergism SERS enhancement effect of the liquid core optical waveguide and the nanometal, namely local electromagnetic enhancement effect of the nanometal, the raman signal strength of surface sample molecules can be greatly improved; meanwhile, through the high light guide performance of the liquid core optical waveguide, more nanoparticles and surface sample molecules inside are chemically enhanced under the action of the excitation light; the high-sensitivity repeatable SERS detection of serum and biological liquid samples can be finally achieved.

Description

technical field [0001] The invention relates to the technical field of micro-nano structures and devices and the technical field of surface-enhanced Raman scattering (SERS) detection. It specifically relates to a new microfluidic SERS detection chip device and a new detection mode for non-destructive detection of blood and biological samples. Background technique [0002] At present, the detection methods commonly used in blood and biological samples include GC / LC-MS, DNA test, immune test and microscopic observation, etc. These detection methods consume a large amount of samples and damage the samples. Existing non-destructive testing methods mainly use ultrasonic testing, ray testing, magnetic testing, acoustic emission testing, laser holographic testing, infrared testing and other acousto-optic testing modes, but these technologies are mainly used in solids with high density such as mechanical devices or bones. detection, and Raman spectroscopy will not cause chemical an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 徐溢王春艳赖春红项松涛陈刚
Owner CHONGQING UNIV